Effects of inhibiting the PI3K/Akt/mTOR signaling pathway on the pain of sciatic endometriosis in a rat model

Yan Liu, Xuying Qin, Xiaofen Lu, Jie Jiang
Canadian journal of physiology and pharmacology · 2019 · vol. 97(10) , pp. 963–970 · doi:10.1139/cjpp-2019-0156 · PMID:31461309 · W2970482736
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Inhibiting the PI3K/Akt/mTOR pathway in a rat model of sciatic endometriosis reduced pain behaviors and decreased expressions of NGF, substance P, and CGRP.

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Abstract

This study investigated the relationship between the pain of sciatic endometriosis and the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway. Adult female Sprague-Dawley rats successfully received sciatic endometriosis induction. Mechanical paw withdrawal threshold and paw withdrawal latency were recorded to assess the mechanical hypersensitivity and thermal hyperalgesia. Quantitative real-time PCR, Western blotting, and enzyme-linked immunosorbent assays were used to detect PI3K, Akt, and mTOR expressions and their phosphorylation as well as the expressions of substance P, calcitonin gene-related peptide (CGRP), and nerve growth factor (NGF). Mechanical paw withdrawal threshold and paw withdrawal latency significantly decreased after sciatic endometriosis induction in rats; this decrease was ameliorated by inhibiting the PI3K/Akt/mTOR signaling pathway using LY294002. Compared with controls, rats with sciatic endometriosis showed increased PI3K, Akt, and mTOR expressions and elevated p-PI3K, p-Akt, and p-mTOR protein expressions. Higher NGF, substance P, and CGRP expressions were also found in the superficial dorsal horn of the spinal cord in rats with sciatic endometriosis than in control rats 21 days after surgery. Following the injection of LY294002 into rats with sciatic endometriosis, there was a significant decrease in the expressions of NGF, substance P, and CGRP. In conclusion, the inhibition of the PI3K/Akt/mTOR signaling pathway may alleviate endometriosis-associated sciatic nerve pain in a rat model of sciatic endometriosis.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Chromones Endometriosis Morpholines Sciatica Sciatic Nerve Signal Transduction Animals Behavior, Animal Behavior, Animal Behavior Observation Techniques Chromones Disease Models, Animal Endometriosis Endometriosis Female Injections, Spinal Morpholines Pain Measurement Phosphatidylinositol 3-Kinases Phosphatidylinositol 3-Kinases

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (37)

Cited by (19)

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