A method for isolating and culturing ectopic epithelial and stromal cells to study human adenomyosis

Fang Zhou, Zhou Fang, Jianzhang Wang, Tiantian Li, Meichen Yin, Yangying Peng, Xinmei Zhang
Archives of gynecology and obstetrics · 2023 · vol. 309(2) , pp. 551–563 · doi:10.1007/s00404-023-07254-8 · PMID:37872452 · W4387880862
article OA: closed CC0 ⤵ 2 in-corpus citations
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⚙ AI-generated summary by claude@2026-06+body, 2026-06-08 ⓘ

This study developed an efficient collagenase digestion and flow cytometry protocol to isolate and culture highly purified ectopic epithelial and stromal cells from human adenomyosis lesions for research.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 ⓘ

This paper studied how to isolate and culture primary ectopic endometrial epithelial and stromal cells directly from human adenomyosis foci, using a collagenase-based digestion approach followed by sterile filtration and flow cytometry-based identification and purification. The authors reported successfully establishing cultures with high purity and viability, including Ep-CAM–expressing ectopic epithelial cells (purity 93.74%, viability 80.58%) and CD10-expressing ectopic stromal cells (cellular purity 96.37%, viability 93.49%). They concluded that the protocol provides a new experimental model to study molecular pathogenesis, with the practical limitation noted by the paper’s focus being that it does not address broader functional/long-term characterization beyond isolation, culture, and marker-based assessment. This paper is centrally about adenomyosis — it develops and validates an efficient protocol for isolating and culturing ectopic epithelial and stromal cells from adenomyosis lesions.

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Condition tags

mesh:D004715adenomyosis

MeSH descriptors

Adenomyosis Adenomyosis Adenomyosis Adenomyosis Endometriosis Endometriosis Endometriosis Endometriosis Endometrium Endometrium Endometrium Endometrium Epithelial Cells Epithelial Cells Epithelial Cells Epithelial Cells Female Female Female Humans

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