Selective Action of N-Arachidonoyl Dopamine on Viability and Proliferation of Stromal Cells from Eutopic and Ectopic Endometrium

We performed a comparative study of the cytotoxic effect of endocannabinoid N-arachidonoyl dopamine (AA-DA) on cultured stromal cells of ectopic and eutopic endometrium. It was found that AA-DA in the concentration range of 1-20 μM produces more selective cytotoxic effect on the stromal cells of the ectopic endometrium due to interaction with cannabinoid type 1 receptor. In concentrations below 1 μM, AA-DA stimulated the proliferation of stromal cells of the eutopic endometrium and did not affect the division of ectopic endometrium cells. This effect was realized due to its interaction with cannabinoid type 2 receptor. Similar content being viewed by others References Endometriosis: Diagnosis, Treatment and Rehabilitation. Federal Clinical Recommendations. Moscow, 2013. Russian. Ashba AM, Akimov MG, Gretskaya NM, Bezuglov VV. N-acyl dopamines induce cell death in PC12 cell line via induction of nitric oxide generation and oxidative stress. Doklady Biochem. Biophysics. 2016;467(1):81-84. Bobrov MY, Lyzhin AA, Andrianova EL, Gretskaya NM, Zinchenko GN, Frumkina LE, Khaspekov LG, Bezuglov VV. Antioxidant and neuroprotective properties of N-docosahexaenoyl dopamine. Bull. Exp. Biol. Med. 2006;142(4):425-427. Chernukha GE, Ilyina LM, Adamyan LV, Pavlovich SV. Deep infiltrating endometriosis: Postoperative recurrences and possible ways of their prevention. Akush. Gin. 2015;(8):39-46. Russian. Akimov MG, Ashba AM, Gretskaya NM, Bezuglov VV. Some aspects of the mechanism of cell death induction by the lipids of N-acyl dopamine family in the PC12 cancer cell line. Anticancer Res. 2014;34(10):5797-5800. Akimov MG, Gretskaya NM, Zinchenko GN, Bezuglov VV. Cytotoxicity of Endogenous Lipids N-acyl Dopamines and their Possible Metabolic Derivatives for human cancer cell lines of different histological origin. Anticancer Res. 2015;35(5):2657-2661. Andrianova EL, Bobrov MY, Gretskaya NM, Zinchenko GN, Fomina-Ageeva EV, Bezuglov VV, Serkov IV. The effects of neurolipins and their synthetic analogues on normal and transformed glial cells. Neurochem. J. 2010;4(1):46-54. Berkley KJ, Rapkin AJ, Papka RE. The pains of endometriosis. Science. 2005;308:1587-1589. Bobrov MY, Lizhin AA, Andrianova EL, Gretskaya NM, Frumkina LE, Khaspekov LG, Bezuglov VV. Antioxidant and neuroprotective properties of N-arachidonoyldopamine. Neurosci. Lett. 2008;431(1):6-11. Giudice LC, Kao LC. Endometriosis. Lancet. 2004;364:1789-1799. Liu X, Yamada N, Maruyama W, Osawa T. Formation of dopamine adducts derived from brain polyunsaturated fatty acids mechanism for Parkinson disease. J. Biol. Chem. 2008; 283(50):34,887-34,895. Schwarz R, Ramer R, Hinz B. Targeting the endocannabinoid system as a potential anticancer approach. Drug Metab. Rev. 2018;50(1):26-53. Sanchez AM, Cioffi R, Viganò P, Candiani M, Verde R, Piscitelli F, Di Marzo V, Garavaglia E, Panina-Bordignon P. Elevated systemic levels of endocannabinoids and related mediators across the menstrual cycle in women with endometriosis. Reprod. Sci. 2016;23(8):1071-1079. Author information Authors and Affiliations Corresponding author Additional information Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 1, pp. 46-50, January, 2019 Rights and permissions About this article Cite this article Ashba, A.M., Yushina, M.N., Fedorova-Gogolina, I.A. et al. Selective Action of N-Arachidonoyl Dopamine on Viability and Proliferation of Stromal Cells from Eutopic and Ectopic Endometrium. Bull Exp Biol Med 167, 43–46 (2019). https://doi.org/10.1007/s10517-019-04456-6 Received: Published: Version of record: Issue date: DOI: https://doi.org/10.1007/s10517-019-04456-6