Smad3/4 Binding to Promoter II of P450arom So As to Regulate Aromatase Expression in Endometriosis

Juan Qu, Yuanyuan Zhu, Xiadi Wu, Juan Zheng, Zhen Hou, Yugui Cui, Yundong Mao, Jiayin Liu
Reproductive sciences (Thousand Oaks, Calif.) · 2016 · vol. 24(8) , pp. 1187–1194 · doi:10.1177/1933719116681517 · PMID:27920344 · W2560811930
article OA: closed CC0 ⤵ 4 in-corpus citations
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⚙ AI-generated summary by claude@2026-06+body, 2026-06-09 ⓘ

Smad3/4 proteins bind to the activin A-responsive P450arom promoter region in endometrial stromal cells, promoting aromatase expression in endometriosis.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 ⓘ

The paper studied how activin A regulates aromatase (P450arom) expression in eutopic endometrial stromal cells (ESCs) from people with endometriosis, focusing on whether Smad3/4 bind promoter II of the CYP19 gene. Using luciferase reporter constructs with serial truncations of promoter II, chromatin immunoprecipitation, and Smad4-targeting siRNA in the presence of activin A, the authors found that Smad3/4 specifically bind to an activin A–responsive region within promoter II and that mutations of predicted Smad binding sites markedly reduced activin A–induced promoter activity; Smad4 knockdown also abolished activin A-driven promoter activation and reduced activin A–dependent p-Smad3 nuclear/cytoplasmic changes. A key limitation is that the functional work is largely based on promoter-reporter and cell-based binding/knockdown assays rather than direct measurement of aromatase protein or in vivo validation. This paper is centrally about endometriosis — it dissects Smad3/4 binding to CYP19 (P450arom) promoter II as a mechanism by which activin A increases aromatase expression in endometriosis-derived ESCs.

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Condition tags

endometriosis

MeSH descriptors

Aromatase Endometriosis Promoter Regions, Genetic Smad3 Protein Smad4 Protein Activins Activins Activins Aromatase Aromatase Endometriosis Endometriosis Endometrium Endometrium Female Humans Smad3 Protein Smad4 Protein Stromal Cells Stromal Cells

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