Induction of the Mice Model Regarding the Endometriosis and Assessment of Antioxidant Treatment Effectiveness: An Experimental Protocol

Hayedeh Hoorsan, Masoumeh Simbar, Fahimeh Ramezani Tehrani, Fardin Fathi, Nariman Mosaffa, Hedyeh Riazi
In: International Journal of Women's Health and Reproduction Sciences · 2020 · vol. 8(2) , pp. 133–141 · doi:10.15296/ijwhr.2020.21 · W3022002311
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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study induced endometriosis in mice to create a model for evaluating vitamin C's antioxidant treatment effectiveness on histological and fertility changes in mothers and their offspring.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 · read from full text ⓘ

This paper describes an experimental protocol to induce endometriosis in mice using both peritoneal and ovarian surgical approaches, with comparison to sham and control groups, followed by assessment of histological and fertility characteristics in first-generation offspring. Across three study phases, 32 mice are randomized to induction vs sham/control, then first-generation outcomes of ovarian endometriosis, sham, and control are compared, and finally vitamin C is administered to evaluate effects on induced endometriosis and its first-generation infants. The study is explicitly framed around literature emphasizing inflammatory, immunologic, and oxidative stress factors in endometriosis, with vitamin C as an antioxidant intervention. This paper is centrally about endometriosis—specifically developing a mouse surgical model (peritoneal and ovarian approaches) and evaluating vitamin C effectiveness on endometriosis progression and reproductive outcomes in first-generation mice.

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Abstract

Objectives : Over the past twenty years, the role of inflammatory, immunologic, and stress oxidative factors in the pathogenesis of endometriosis has been emphasized in the literature. Endometrial induced lesions in the surgical endometriosis model of mice are similar to those observed in women. Therefore, the present study evaluated the effects of vitamin C treatment on the endometriosis mice model. More precisely, it aimed to induce an optimum model of endometriosis using both peritoneal and ovarian approaches and to compare the histological and fertility changes in the first-generation infants of endometriosis induced, sham, and control groups. Finally, the study assessed the influence of vitamin C administration treatment on the induced endometriosis mice model and its first-generation infants. Methods : This experimental study was done in three phases. In the first phase, 32 mice were randomly divided into four groups receiving induced endometriosis through peritoneal approach, induced endometriosis through an ovarian approach, as well as sham and control groups. The histological and fertility characteristics of the first generation of ovarian endometriosis, sham, and control groups were compared in the second phase. Then, the histological and fertility characteristics of those endometriosis induced models receiving vitamin C were compared with the controls, and the first generation of these groups was compared as well. Results : This experimental study began in April 2017 and will be finished in 2020. Conclusions : The results of this study improve our knowledge about the mechanisms involved in endometriosis progressions. The results further provide a comprehensive animal model for further research and assess the therapeutic role of antioxidants.
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Apr 2020, Vol 8, Issue 2 Advanced Search For Author's & Reviewer's Poll How do you find the scientific quality of the published articles on our web site? | Study Protocol | | | Induction of the Mice Model Regarding the Endometriosis and Assessment of Antioxidant Treatment Effectiveness: An Experimental Protocol | | | Hayedeh Hoorsan1, Masoumeh Simbar2, Fahimeh Ramezani Tehrani3, Fardin Fathi4, Nariman Mosaffa5, Hedyeh Riazi6 | | | 1Student Research Committee, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Science, Tehran, Iran 3Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran 4Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran 5Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 6Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran | | | IJWHR 2020; 8: 133-141 DOI: 10.15296/ijwhr.2020.21 Viewed : 3677 times Downloaded : 3079 times. Keywords : Model of endometriosis, Mice, First-generation, Antioxidant treatment | | | Full Text(PDF) | Related Articles | | | Abstract | | Objectives: Over the past twenty years, the role of inflammatory, immunologic, and stress oxidative factors in the pathogenesis of endometriosis has been emphasized in the literature. Endometrial induced lesions in the surgical endometriosis model of mice are similar to those observed in women. Therefore, the present study evaluated the effects of vitamin C treatment on the endometriosis mice model. More precisely, it aimed to induce an optimum model of endometriosis using both peritoneal and ovarian approaches and to compare the histological and fertility changes in the first-generation infants of endometriosis induced, sham, and control groups. Finally, the study assessed the influence of vitamin C administration treatment on the induced endometriosis mice model and its first-generation infants. Methods: This experimental study was done in three phases. In the first phase, 32 mice were randomly divided into four groups receiving induced endometriosis through peritoneal approach, induced endometriosis through an ovarian approach, as well as sham and control groups. The histological and fertility characteristics of the first generation of ovarian endometriosis, sham, and control groups were compared in the second phase. Then, the histological and fertility characteristics of those endometriosis induced models receiving vitamin C were compared with the controls, and the first generation of these groups was compared as well. Results: This experimental study began in April 2017 and will be finished in 2020. Conclusions: The results of this study improve our knowledge about the mechanisms involved in endometriosis progressions. The results further provide a comprehensive animal model for further research and assess the therapeutic role of antioxidants. | Cite By, Google Scholar Articles by Hoorsan H Articles by Simbar M Articles by Ramezani Tehrani F Articles by Fathi F Articles by Mosaffa N Articles by Riazi H Articles by Hayedeh Hoorsan Articles by Masoumeh Simbar Articles by Fahimeh Ramezani Tehrani Articles by Fardin Fathi Articles by Nariman Mosaffa Articles by Hedyeh Riazi Submit Paper Online Submission System IJWHR ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal Online Submission System IJWHR ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal Publication Information Publisher Aras Part Medical International Press Editor-in-Chief Arash Khaki Mertihan Kurdoglu Deputy Editor Zafer Akan Aras Part Medical International Press Editor-in-Chief Arash Khaki Mertihan Kurdoglu Deputy Editor Zafer Akan Published Article Statistics

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