Analysis of DNA methylation level and mRNA expression of Transient Receptor Ankyrin Member 1 (TRPA1) in endometriosis-associated pain

Ocktariyana Ocktariyana, Nurul Hikmawati, Andon Hestiantoro, Raden Muharam, M. Luky Satria Marwali, Agus Surur Asadi, Tiara Aninditha, Gita Pratama, Anisah Ainun Zahrah, Nabilla Farah Naura, Asmarinah Asmarinah
In: Asia Pacific Journal of Molecular Biology and Biotechnology · 2021 · pp. 1–10 · doi:10.35118/apjmbb.2021.029.3.01 · W3174001866
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⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

This study analyzed TRPA1 DNA methylation and mRNA expression in endometriosis, finding significantly higher TRPA1 mRNA in peritoneal endometriosis but no differences in DNA methylation or correlations with pain.

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Abstract

Transient Receptor Ankyrin Member 1 (TRPA1) is an ion channel family protein that regulates pain sensation through sensory neurons' activity. This study's purpose to analyzes the DNA methylation and mRNA expression level of the TRPA1 gene in endometriosis and its correlation with pain level. Twenty samples of peritoneal endometriosis and endometrial samples were obtained from women with endometriosis, which was subsequently compared to 20 endometrial samples of women without endometriosis. The DNA methylation level of TRPA1 was analyzed using Methylation-specific PCR (MS-PCR) and ImageJ software, while the mRNA expression of TRPA1 was analyzed using qRT-PCR. Furthermore, the pain level was measured using the numeric rating scale (NRS) by interviewing all the women. This study showed that there was a significant difference in the mRNA expression of TRPA1 in peritoneal endometriosis. The TRPA1 was unmethylated in both peritoneal and endometrial samples in endometriosis. However, DNA Methylation level of TRPA1 in peritoneal and endometrial of endometriosis compared to normal endometrial were no significant difference. Additionally, there was no correlation between DNA methylation level and mRNA expression level of TRPA1 in all samples, along with the endometriosis-associated pain.

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Outcome instruments

NRS-pain

Condition tags

endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (38)

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