DNA methylation of P2X3 receptor gene encoded pain marker protein in endometriosis

Ocktariyana Ocktariyana, Andon Hestiantoro, R. Febri Rahmala, Asmarinah Asmarinah
In: Journal of Physics: Conference Series · 2019 · vol. 1246(1) , pp. 012031 · doi:10.1088/1742-6596/1246/1/012031 · W2962528374
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This study found that the P2X3 receptor gene promoter was 100% unmethylated in both endometriosis and control tissues, with no significant differences between groups, despite increased P2X3 receptor expression in endometriosis patients.

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This study investigated the DNA methylation status of the P2X3 receptor gene promoter in peritoneal endometriotic tissue from nine patients compared to control endometrial tissue from nine women without the condition. Using methyl-specific PCR and gel electrophoresis, researchers found that both groups exhibited 100% unmethylated promoters with no statistically significant difference between them, despite a higher density of unmethylated bands in the patient group. The authors noted a significant correlation between pain and endometriosis but concluded that the lack of methylation differences suggests this specific epigenetic marker may not be reliable for diagnosis. This paper is centrally about endometriosis — specifically analyzing genetic markers associated with pain mechanisms in peritoneal lesions.

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Abstract

Abstract Endometriosis is a common, benign, oestrogen-dependent, chronic gynaecological disorder associated with pelvic pain and infertility. Increasing of P2X3 receptors induced sensitization of pain in endometriosis patients. Epigenetic mechanism such as DNA methylation could lead to alteration of gene expression. The aim of this study was to analyse DNA methylation of P2X3 receptor gene promoter in peritoneal endometriotic tissue from 9 patients compared to endometrial tissue from 9 without endometriosis women as control. The DNA from samples was isolated and with sodium bisulfite converted. We used Methyl Specific PCR (MSP) method to amplify the DNA and then running MSP product in gel electrophoresis. The band intensity of samples were measured by ImageJ software. Statistical analysis was significant correlation between pain and endometriosis (p=0.000). DNA methylation of P2X3 receptor gene promoter among peritoneal endometriotic tissue in women endometriosis and endometrial tissue woman without endometriosis were 100% unmethylated and there was no significant differences (p=0.287), although density of band unmethylated peritoneum endometriosis group was higher than control group. This study was suggesting that DNA methylation of P2X3 receptor gene promoter might be a potential biomarker to early diagnostic of endometriosis without invasive procedure in endometriosis patienst especially with pain symptoms.
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Abstract Endometriosis is a common, benign, oestrogen-dependent, chronic gynaecological disorder associated with pelvic pain and infertility. Increasing of P2X3 receptors induced sensitization of pain in endometriosis patients. Epigenetic mechanism such as DNA methylation could lead to alteration of gene expression. The aim of this study was to analyse DNA methylation of P2X3 receptor gene promoter in peritoneal endometriotic tissue from 9 patients compared to endometrial tissue from 9 without endometriosis women as control. The DNA from samples was isolated and with sodium bisulfite converted. We used Methyl Specific PCR (MSP) method to amplify the DNA and then running MSP product in gel electrophoresis. The band intensity of samples were measured by ImageJ software. Statistical analysis was significant correlation between pain and endometriosis (p=0.000). DNA methylation of P2X3 receptor gene promoter among peritoneal endometriotic tissue in women endometriosis and endometrial tissue woman without endometriosis were 100% unmethylated and there was no significant differences (p=0.287), although density of band unmethylated peritoneum endometriosis group was higher than control group. This study was suggesting that DNA methylation of P2X3 receptor gene promoter might be a potential biomarker to early diagnostic of endometriosis without invasive procedure in endometriosis patienst especially with pain symptoms. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

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endometriosisinfertility

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