Cellular heterogeneity in long-term surviving cells isolated from eutopic endometrial, ovarian endometrioma and adenomyosis tissues

Tetsuji Tanaka, Satoko Nakajima, Naohiko Umesaki
Oncology reports · 2003 · vol. 10(5) , pp. 1155–60 · doi:10.3892/or.10.5.1155 · PMID:12883673 · W2089056364
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

Eutopic endometrial, endometrioma, and adenomyosis cells were cultured, revealing distinct long-term surviving monoclonal cell types from eutopic endometrium and demonstrating that dispersed endometrial cells, not tissue fragments, fail to induce endometriosis in mice.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 · read from full text ⓘ

The study investigated whether stem-like cells can survive and proliferate long term by performing limiting dilution cultures of cells from one patient’s eutopic endometrium, ovarian endometrioma, and adenomyosis tissues, and then establishing monoclonal cultures. Cloning efficiencies were 13.0% for eutopic endometrium, 5.0% for ovarian endometrioma, and 0.8% for adenomyosis, and the resulting monoclonal cells survived for more than 24 months, with multiple distinct types derived from eutopic endometrial cells. In scid mice, dispersed human eutopic endometrial cells failed to produce endometriosis-like lesions, whereas endometrial tissue fragments did, and tested cytokines (IL-6, TNF-α, IL-1β, M-CSF, HGF) did not enhance engraftment of dispersed cells. This paper centrally about endometriosis — it uses eutopic endometrial, ovarian endometrioma, and adenomyosis tissues to show heterogeneous long-term surviving cell types and that dispersed “stem-like” cells alone may not induce peritoneal endometriosis.

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Abstract

Human endometrial tissues regenerate easily after menstruation and childbirth, suggesting the existence of endometrial stem-like cells that can survive and proliferate from a single cell over a long time. To clarify this hypothesis, limiting dilution cultures performed with eutopic endometrial, ovarian endometrioma and adenomyosis cells obtained from a patient, achieved cloning efficiencies of 13.0, 5.0, and 0.8%, respectively. These monoclonal cells survived for more than 24 months. More than 4 types of monoclonal cells were established from eutopic endometrial cells and microscopically were distinctly different from each other. Intraperitoneal injections of dispersed human eutopic endometrial cells did not cause any endometriosis-like lesions in scid mice, but those of endometrial tissue fragments did. IL-6, TNF-alpha, IL-1beta, M-CSF and HGF failed to enhance transplantation of dispersed endometrial cells to the mice. These results indicate that several types of eutopic endometrial cells survive long-term, and that simple regurgitation of eutopic endometrial stem-like cells may not induce peritoneal endometriosis.
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Print ISSN: 1021-335X Online ISSN: 1791-2431 International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease. International Journal of Oncology is an international journal devoted to oncology research and cancer treatment. Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery. Oncology Reports is an international journal devoted to fundamental and applied research in Oncology. Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine. Oncology Letters is an international journal devoted to Experimental and Clinical Oncology. Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology. International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis. Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology. Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition. Publishes open-access research on using epigenetics to advance understanding and treatment of human disease. An International Open Access Journal Devoted to General Medicine. Article - Authors: - Pages: 1155-1160|Published online on: September 1, 2003https://doi.org/10.3892/or.10.5.1155 - Expand metrics + Human endometrial tissues regenerate easily after menstruation and childbirth, suggesting the existence of endometrial stem-like cells that can survive and proliferate from a single cell over a long time. To clarify this hypothesis, limiting dilution cultures performed with eutopic endometrial, ovarian endometrioma and adenomyosis cells obtained from a patient, achieved cloning efficiencies of 13.0, 5.0, and 0.8%, respectively. These monoclonal cells survived for more than 24 months. More than 4 types of monoclonal cells were established from eutopic endometrial cells and microscopically were distinctly different from each other. Intraperitoneal injections of dispersed human eutopic endometrial cells did not cause any endometriosis-like lesions in scid mice, but those of endometrial tissue fragments did. IL-6, TNF-α, IL-1β, M-CSF and HGF failed to enhance transplantation of dispersed endometrial cells to the mice. These results indicate that several types of eutopic endometrial cells survive long-term, and that simple regurgitation of eutopic endometrial stem-like cells may not induce peritoneal endometriosis. Copy and paste a formatted citation Spandidos Publications style Tanaka T, Nakajima S and Umesaki N: Cellular heterogeneity in long-term surviving cells isolated from eutopic endometrial, ovarian endometrioma and adenomyosis tissues. Oncol Rep 10: 1155-1160, 2003. APA Tanaka, T., Nakajima, S., & Umesaki, N. (2003). Cellular heterogeneity in long-term surviving cells isolated from eutopic endometrial, ovarian endometrioma and adenomyosis tissues. Oncology Reports, 10, 1155-1160. https://doi.org/10.3892/or.10.5.1155 MLA Tanaka, T., Nakajima, S., Umesaki, N."Cellular heterogeneity in long-term surviving cells isolated from eutopic endometrial, ovarian endometrioma and adenomyosis tissues". Oncology Reports 10.5 (2003): 1155-1160. Chicago Tanaka, T., Nakajima, S., Umesaki, N."Cellular heterogeneity in long-term surviving cells isolated from eutopic endometrial, ovarian endometrioma and adenomyosis tissues". Oncology Reports 10, no. 5 (2003): 1155-1160. https://doi.org/10.3892/or.10.5.1155

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Condition tags

endometriosisadenomyosisendometrioma

MeSH descriptors

Endometrial Neoplasms Endometriosis Ovarian Neoplasms Adult Animals Cell Division Cell Survival Endometrial Neoplasms Endometrial Neoplasms Endometriosis Endometriosis Female Humans Immunohistochemistry Mice Mice, SCID Ovarian Neoplasms Ovarian Neoplasms Time Factors

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