Clinical usefulness of E4-phytohemagglutinin reactive C4-binding protein relative front value in serum for distinguishing between ovarian clear cell adenocarcinoma and endometrioma

Shimizu K, Kayoko Shimizu, Sakamoto T, Tomotaka Sakamoto, Toshio Ohashi, Ohashi T, Toshinari Ohashi, Tanabe K, Kazuhiro Tanabe, Io Hayashi, Hayashi I, Ikeda M, Masae Ikeda, Mikio Mikami
Clinica chimica acta; international journal of clinical chemistry · 2025 · vol. 571 , pp. 120219 · doi:10.1016/j.cca.2025.120219 · PMID:40024277 · W4408031381
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Serum E4-phytohemagglutinin-reactive C4BP relative front value was significantly higher in ovarian clear cell adenocarcinoma than in controls and aided differentiation from endometrioma, especially when combined with HE4.

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Abstract

BACKGROUND AND AIMS: We identified a fully sialylated glycopeptide of the α-chain of Complement 4 binding protein (C4BP) in blood using Liquid chromatography/Mass spectrometry, which helps differentiate ovarian clear cell carcinoma (OCCC) from endometrioma (EM). We aimed to develop a blood measurement system to immunologically assess fully sialylated α-chain of C4BP, specifically E4-phytohemagglutinin-reactive C4BP (P-C4BP-Rf). MATERIALS AND METHODS: Ninety-seven serum samples were collected from healthy (HE) (n = 11), uterine fibroid (UF) patients (n = 15), EM patients (n = 32), and OCCC patients (n = 39). P-C4BP-Rf was measured using lectin affinity electrophoresis and antibody affinity blotting. CA125 and human epididymal protein 4 (HE4) levels were also measured and compared. We selected 22 EM and 24 OCCC with no significant differences in CA125 levels. Tissue factor pathway inhibitor 2 (TFPI2) and FS-C4BP were added to the analysis to distinguish OCCC from EM using five biomarkers. RESULTS: P-C4BP-Rf was significantly higher in OCCC than in HE, UF, and EM (P < 0.0001). AUC values were: CA125, 0.547; HE4, 0.850; TFPI2, 0.847; FS-C4BP, 0.715; and P-C4BP-Rf, 0.887. The combination of P-C4BP-Rf and HE4 yielded the highest AUC (0.960). CONCLUSION: P-C4BP-Rf is useful for distinguishing OCCC from EM and may be more accurate when combined with HE4.

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Condition tags

endometriosisendometrioma

MeSH descriptors

Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (28)

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