Metformin attenuates expression of angiogenic and inflammatory genes in human endometriotic stromal cells

Simin Yari, Yari S, Khoei HH, Heidar Heidari Khoei, Saber M, Maryam Saber, Fereshteh Esfandiari, Esfandiari F, Ashraf Moini, Moini A, Shahhoseini M, Maryam Shahhoseini
Experimental cell research · 2021 · vol. 404(2) , pp. 112659 · doi:10.1016/j.yexcr.2021.112659 · PMID:34022204 · W3163766363
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Metformin was found to reduce the expression of genes related to angiogenesis and inflammation in human endometriotic stromal cells.

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Abstract

Endometriosis is a benign gynecological disease that is manifested by the presence and growth of endometrial cells and glands outside the uterine. Active angiogenesis, migration, and invasion of endometrial tissue outside the uterine are critical for the development of endometriosis and lead to the survival and growth of endometriotic lesions. Metformin, as an anti-diabetic agent, represents anti-angiogenic property. Here, we performed a study using human normal endometrial stromal cells (N-ESCs) from healthy endometrial tissue and human eutopic endometrial stromal cells (EU-ESCs) and ectopic endometrial stromal cells (ECT-ESCs) from endometriosis patients. ESCs were cultured and treated with different concentrations of Metformin (0-20 mmol/l) for 72 h to evaluate Metformin effect on cell viability, proliferation, migration was measured by methyl thiazolyl tetrazolium (MTT) assay and scratch test respectively as well as expression of angiogenesis and migration markers. The Metformin reduced cell migration, and proliferation of endometriotic stromal cells in a time and concentration dependently manner. Furthermore, Metformin attenuated the expression of angiogenic and inflammatory genes in human endometriotic stromal cells. The direct anti-proliferative effect on ECT-ESCs combined with the effects of Metformin on inflammatory and angiogenesis-related genes expression supports its therapeutic potential for endometriosis. Metformin could be used as an effective adjuvant in endometriosis treatment.

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Condition tags

endometriosis

MeSH descriptors

Endometrium Metformin Neovascularization, Pathologic Stromal Cells Cell Movement Cell Movement Cell Movement Cell Proliferation Cell Proliferation Cell Proliferation Cells, Cultured Endometriosis Endometriosis Endometriosis Endometrium Endometrium Epithelial Cells Epithelial Cells Epithelial Cells Female

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