Progestins regulate the expression and activity of the forkhead transcription factor FOXO1 in differentiating human endometrium

Soraya Labied, Takeshi Kajihara, Patricia A Madureira, Patrícia A. Madureira, Luca Fusi, Marius C. Jones, Jenny Higham, Jenny M Higham, Rana Varshochi, Julia M. Francis, Georgia Zoumpoulidou, Abdelkader Essafi, Silvia Fernandez de Mattos, Silvia Fernández de Mattos, Eric W-F Lam, Eric W.‐F. Lam, Jan J. Brosens
Molecular Endocrinology · 2005 · vol. 20(1) , pp. 35–44 · doi:10.1210/me.2005-0275 · PMID:16123151 · W1999084704
Lab / animal OA: bronze CC0 ⤵ 19 in-corpus citations
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This study identifies FOXO1 as a progestin-regulated transcription factor that mediates apoptosis in differentiating human endometrial stromal cells upon progesterone withdrawal, linking decidualization to menstruation.

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Abstract

Menstruation, or cyclic shedding of nonpregnant endometrial tissue with associated bleeding, occurs only in humans and a few other species. This breakdown of the endometrium in response to falling ovarian progesterone levels is a complex process, characterized by local leukocyte infiltration, expression and activation of matrix metalloproteinases, and apoptosis. Spontaneous decidualization (differentiation) of the stromal compartment precedes the cyclic shedding of the endometrium in various menstruating species but the mechanisms that link these processes are not understood. In this study, we identified FOXO1 as a key transcription factor responsible for mediating apoptosis of decidualized human endometrial stromal cells (HESCs) in response to progesterone withdrawal. We demonstrate that medroxyprogesterone acetate (MPA, a synthetic progestin) enhances the expression of FOXO1 in differentiating HESCs while simultaneously inducing cytoplasmic retention and inactivation of FOXO1. Withdrawal of MPA from decidualized HESCs results in rapid nuclear accumulation of FOXO1, increased BIM expression, a proapoptotic FOXO1 target gene, and cell death. Conversely, silencing of FOXO1 expression completely abolishes cell death induced by MPA withdrawal. In summary, the observation that differentiating HESCs become dependent on progesterone signaling for survival through induction and reversible inactivation of FOXO1 suggests a novel mechanism that links decidualization of the endometrium to menstruation.

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MeSH descriptors

Endometrium Forkhead Transcription Factors Progestins Active Transport, Cell Nucleus Apoptosis Regulatory Proteins Apoptosis Regulatory Proteins Bcl-2-Like Protein 11 Cell Differentiation Cells, Cultured Cyclic AMP Cyclic AMP Cytoplasm Cytoplasm Decidua Decidua Decidua Endometrium Endometrium Female Forkhead Box Protein O1

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