P53 and Murine Double Mimute 2 (MDM2) Expression Changes and Significance in Different Types of Endometrial Lesions

Jiang Z, Zhongyong Jiang, Wanqing Xu, Xu W, Dan G, Gang Dan, Liu Y, Yuan Liu, Xiong J, Jie Xiong
Medical science monitor : international medical journal of experimental and clinical research · 2016 · vol. 22 , pp. 4786–4793 · doi:10.12659/msm.898616 · PMID:27924072 · PMC5158131 · W2559954938
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This study found elevated p53 and MDM2 expression in endometrial polyps and adenocarcinoma, with increased levels correlating to higher clinical stages of adenocarcinoma.

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This study compared p53 and MDM2 mRNA and protein expression across normal endometrium, endometrial polyps, uterine adenomyosis, and endometrial adenocarcinoma, using real-time PCR, immunohistochemistry, and Western blotting, and assessed associations with endometrial adenocarcinoma clinical stage. p53 and MDM2 expression were significantly elevated in endometrial polyps and endometrial adenocarcinoma versus normal controls, with higher levels in adenocarcinoma than polyps, and p53 and MDM2 levels were positively correlated. In uterine adenomyosis, p53 and MDM2 were only slightly increased and did not reach statistical significance relative to controls, and higher expression was observed with more advanced adenocarcinoma staging (stage III vs II, and II vs I). The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

BACKGROUND Endometrial lesions are common in obstetrics and gynecology, including endometrial polyps, uterine adenomyosis, and malignant endometrial adenocarcinoma. Endometrial lesions seriously affect women's health, fertility, quality of life, and life safety. As a pro-apoptosis gene, p53 is considered to be closely related with human tumors. Murine double mimute 2 (MDM2) is an oncogene that can promote tumor occurrence and development. P53 and MDM2 expression and significance in different types of endometrial lesions have not been fully elucidated. MATERIAL AND METHODS Normal endometrium, endometrial polyps, uterine adenomyosis, and endometrial adenocarcinoma tissue samples were collected. Real-time PCR was used to detect p53 and MDM2 mRNA expression. Immunohistochemical staining and Western blot analysis were applied to test p53 and MDM2 protein expression. Their correlation with clinical staging of endometrial adenocarcinoma was analyzed. RESULTS P53 and MDM2 mRNA and protein expression were significantly elevated in the endometrial polyps group and the endometrial adenocarcinoma group compared with the normal control group (P<0.05). Their levels increased more obviously in endometrial adenocarcinoma compared with endometrial polyps (P0.05). P53 and MDM2 mRNA and protein level showed a positive correlation. Significantly higher expression of p53 or MDM2 was observed in patients with stage III compared to those in patients with stage II. Higher expression was also observed in patients with stage II than in patients with stage I. CONCLUSIONS P53 and MDM2 mRNA and protein were elevated in endometrial polyps and endometrial adenocarcinoma and their expressions were correlated with clinical staging of endometrial adenocarcinoma. They can promote cancer occurrence and development, and can be treated to assist diagnosis and provide a reference for treatment.
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Abstract

BACKGROUND: Endometrial lesions are common in obstetrics and gynecology, including endometrial polyps, uterine adenomyosis, and malignant endometrial adenocarcinoma. Endometrial lesions seriously affect women’s health, fertility, quality of life, and life safety. As a pro-apoptosis gene, p53 is considered to be closely related with human tumors. Murine double mimute 2 (MDM2) is an oncogene that can promote tumor occurrence and development. P53 and MDM2 expression and significance in different types of endometrial lesions have not been fully elucidated.

Material and methods

Normal endometrium, endometrial polyps, uterine adenomyosis, and endometrial adenocarcinoma tissue samples were collected. Real-time PCR was used to detect p53 and MDM2 mRNA expression. Immunohistochemical staining and Western blot analysis were applied to test p53 and MDM2 protein expression. Their correlation with clinical staging of endometrial adenocarcinoma was analyzed.

Results

P53 and MDM2 mRNA and protein expression were significantly elevated in the endometrial polyps group and the endometrial adenocarcinoma group compared with the normal control group (P<0.05). Their levels increased more obviously in endometrial adenocarcinoma compared with endometrial polyps (P0.05). P53 and MDM2 mRNA and protein level showed a positive correlation. Significantly higher expression of p53 or MDM2 was observed in patients with stage III compared to those in patients with stage II. Higher expression was also observed in patients with stage II than in patients with stage I.

Conclusions

P53 and MDM2 mRNA and protein were elevated in endometrial polyps and endometrial adenocarcinoma and their expressions were correlated with clinical staging of endometrial adenocarcinoma. They can promote cancer occurrence and development, and can be treated to assist diagnosis and provide a reference for treatment.

Keywords

Adenomyosis - pathology, Adenocarcinoma - pathology, Diagnosis, Differential, Endometrial Neoplasms - pathology, Polyps - pathology, Proto-Oncogene Proteins c-mdm2 - genetics, Quality of Life, RNA, Messenger - metabolism, Tumor Suppressor Protein p53 - genetics Editorial 01 January 2026 : Editorial Editorial: Increasing Awareness of Lung Cancer in Non-Smokers and Never-Smokers Challenges Current Approaches to Prevention and ScreeningDOI: 10.12659/MSM.952454 Med Sci Monit 2026; 32:e952454 In Press Clinical Research Institutional and Regional Variations in Access to Clinical Trials and Next-Generation Sequencing in Turkis...Med Sci Monit In Press; DOI: 10.12659/MSM.951027 Clinical Research Low-Intensity Blood Flow-Restricted Multi-Joint Exercise Improves Muscle Function in Patients With Patellof...Med Sci Monit In Press; DOI: 10.12659/MSM.950516 Review article Musculoskeletal Ultrasound and MRI in the Evaluation of Chemotherapy-Induced Peripheral Neuropathy: A ReviewMed Sci Monit In Press; DOI: 10.12659/MSM.951283 Clinical Research Sensory Processing, Dissociation, and Affective Symptoms in Misophonia: A Cross-Sectional Study of 35 AdultsMed Sci Monit In Press; DOI: 10.12659/MSM.950938 Most Viewed Current Articles 17 Jan 2024 : Review article 10,187,196 Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799 Med Sci Monit 2024; 30:e942799 13 Nov 2021 : Clinical Research 3,708,487 Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788 Med Sci Monit 2021; 27:e932788 14 Dec 2022 : Clinical Research 2,341,643 Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990 Med Sci Monit 2022; 28:e937990 16 May 2023 : Clinical Research 706,524 Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387 Med Sci Monit 2023; 29:e940387

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Condition tags

adenomyosis

MeSH descriptors

Endometrial Neoplasms Proto-Oncogene Proteins c-mdm2 Tumor Suppressor Protein p53 Adenocarcinoma Adenocarcinoma Adenocarcinoma Adenocarcinoma Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adult Aged Diagnosis, Differential Endometrial Neoplasms Endometrial Neoplasms Endometrial Neoplasms Female Gene Expression Regulation, Neoplastic Humans

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