The Difference of Bax Protein Expression between Endometrioma and Ovarian Carcinoma

Chandran Frinaldo Saragih, Riza Rivany, M. Fauzie Sahil, Fadjrir Fadjrir, Edy Ardiansyah, Muhammad Rizki Yaznil, Munauwarus Sarirah
In: Molecular and Cellular Biomedical Sciences · 2019 · vol. 3(2) , pp. 95 · doi:10.21705/mcbs.v3i2.50 · W2970001970
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This study examined Bax protein expression in endometrioma and ovarian carcinoma tissues using immunohistochemistry and found no statistically significant difference between the two.

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This study compared Bax protein expression between endometrioma tissues and ovarian carcinoma tissues (serous, mucinous, clear cell, and endometrioid types) by immunohistochemistry on 50 paraffin blocks using a monoclonal antibody and automated staining (Bondmax). Bax expression was scored and the authors tested differences in expression scores between groups and across ovarian cancer clinical stages and histopathological types using the Mann–Whitney test. The mean Bax expression scores were 3.88 for endometrioma and 3.72 for ovarian carcinoma, with no statistically significant difference between them (p>0.05), and no association with ovarian cancer stage or histological subtype (p>0.05). This paper is centrally about endometriosis — specifically, it analyzes Bax protein expression differences between endometrioma (endometriosis-associated cysts) and ovarian carcinoma.

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Abstract

Background: Endometriosis is a benign disease that has malignant properties such as genetic polymorphism, loss control of cell proliferation, infiltration, and local spread or to distant places. Several endometriosis studies linking endometrioma/ ovarian endometriosis with an increased risk of ovarian malignancy give rise to a transformation phenomenon of endometriotic cysts into malignancy. Bax is a pro apoptotic protein whose expression decreases in a malignancy. This decrease is related to the poor prognosis of endometrioma and ovarian carcinoma. This study was aimed to identify the expression and the difference of Bax expression between endometrioma and ovarian carcinoma.Materials and Methods: Fifty of paraffin blocks of endometrioma tissue and ovarian carcinoma (serous, mucinous, clear cell, and endometrioid type) were examined by immunohistochemical using Bondmax Full Automatic with specific monoclonal antibody to identify Bax expression. The difference of Bax expression score between endometrioma tissue and ovarian carcinoma was tested by Mann-Whitney test with significant value was set at p<0.05.Results: This study found that mean Bax expression score in endometrioma tissue and ovarian carcinoma was 3.88 and 3.72. No difference of Bax expression between endometrioma tissue and ovarian carcinoma (p>0.05). No difference of Bax expression between the clinical stages and histopathological types of ovarian carcinoma (p>0.05).Conclusion: There are no statistically significant difference in Bax protein expression in ovarian cancer and endometrioma.Keywords: Bax expression, endometrioma, ovarian carcinoma, apoptotic resistance
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Abstract

Background: Endometriosis is a benign disease that has malignant properties such as genetic polymorphism, loss control of cell proliferation, infiltration, and local spread or to distant places. Several endometriosis studies linking endometrioma/ ovarian endometriosis with an increased risk of ovarian malignancy give rise to a transformation phenomenon of endometriotic cysts into malignancy. Bax is a pro apoptotic protein whose expression decreases in a malignancy. This decrease is related to the poor prognosis of endometrioma and ovarian carcinoma. This study was aimed to identify the expression and the difference of Bax expression between endometrioma and ovarian carcinoma.

Materials and methods

Fifty of paraffin blocks of endometrioma tissue and ovarian carcinoma (serous, mucinous, clear cell, and endometrioid type) were examined by immunohistochemical using Bondmax Full Automatic with specific monoclonal antibody to identify Bax expression. The difference of Bax expression score between endometrioma tissue and ovarian carcinoma was tested by Mann-Whitney test with significant value was set at p<0.05.

Results

This study found that mean Bax expression score in endometrioma tissue and ovarian carcinoma was 3.88 and 3.72. No difference of Bax expression between endometrioma tissue and ovarian carcinoma (p>0.05). No difference of Bax expression between the clinical stages and histopathological types of ovarian carcinoma (p>0.05).

Conclusion

There are no statistically significant difference in Bax protein expression in ovarian cancer and endometrioma.

Keywords

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Reduced expression of BAX is associated with poor prognosis in patients with epithelial ovarian cancer: a multifactorial analysis of TP53, p21, BAX andBCL-2. Br J Cancer. 2001; 85(9): 1359-67, CrossRef. DOI: https://doi.org/10.21705/mcbs.v3i2.50 Copyright (c) 2019 Cell and BioPharmaceutical Institute This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. Indexed by: The Prodia Education and Research Institute

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