Endometrial stromal cell proteomic analysis reveals LIM and SH3 protein 1 (LASP1) plays important roles in the progression of adenomyosis

Faying Liu, Liu F, Zengming Li, Li Z, Jiu-Bai Guo, Guo J, Jiubai Guo, Shu-Fen Fang, Fang S, Shufen Fang, Zhou J, Jiangyan Zhou, Cao B, Bianna Cao, Liu J, Jun Liu, Yulan Yi, Yi Y, Xiao-Qun Yuan, Xiaoqun Yuan, Yuan X, Xiaoyun Xu, Xu X, Ouping Huang, Huang O, Wang L, Liqun Wang, Yang Zou, Zou Y
Molecular human reproduction · 2021 · vol. 27(3) · doi:10.1093/molehr/gaab008 · PMID:33543750 · W3127874012
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Proteomic analysis of adenomyosis endometrial stromal cells revealed increased LIM and SH3 protein 1 (LASP1) expression, which enhances cell proliferation, migration, and invasion and is associated with promoter DNA hypermethylation.

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Abstract

Adenomyosis is one of the most common gynecological disorders that the molecular events underlying its pathogenesis remain not fully understood. Prior studies have shown that endometrial stromal cells (ESCs) played crucial roles in the pathogenesis of adenomyosis. In this study, we utilized two-dimensional gel electrophoresis combined with protein identification by mass spectrometry (2D/MS) proteomics analysis to compare the differential protein expression profile between the paired eutopic and ectopic ESCs (EuESCs and EcESCs) in adenomyosis, and a total of 32 significantly altered protein spots were identified. Among which, the expression of LIM and SH3 protein 1 (LASP1) was increased significantly in EcESCs compared to EuESCs. Immunohistochemical assay showed that LASP1 was overexpressed in the stromal cells of ectopic endometriums compared to eutopic endometriums; further functional analyses revealed that LASP1 overexpression could enhance cell proliferation, migration and invasion of EcESCs. Furthermore, we also showed that the dysregulated expression of LASP1 in EcESCs was associated with DNA hypermethylation in the promoter region of the LASP1 gene. However, the detailed molecular mechanisms of enhancing cell proliferation, invasion and migration caused by upregulated LASP1 in adenomyosis needs further study. For the first time, our data suggested that LASP1 plays important roles in the pathogenesis of adenomyosis, and could serve as a prognostic biomarker of adenomyosis.

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Condition tags

adenomyosis

MeSH descriptors

Adaptor Proteins, Signal Transducing Adenomyosis Cytoskeletal Proteins Endometrium LIM Domain Proteins Proteome Proteome Proteomics Proteomics Stromal Cells Adaptor Proteins, Signal Transducing Adaptor Proteins, Signal Transducing Adaptor Proteins, Signal Transducing Adenomyosis Adenomyosis Adenomyosis Adenomyosis Case-Control Studies Case-Control Studies Cell Proliferation

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