Differential immunohistochemical expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in cow uteri with adenomyosis during follicular phase

Lio Moreira, Moreira L, de Carvalho EC, Eulógio Carlos Queiroz de Carvalho, Maria Clara Caldas-Bussiere, M.C. Caldas-Bussiere
Veterinary research communications · 2011 · vol. 35(5) , pp. 261–269 · doi:10.1007/s11259-011-9470-1 · PMID:21626345 · W2112580893
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Matrix metalloproteinase-2 expression was higher in deep adenomyosis cow uteri during the follicular phase compared to tissue inhibitor of metalloproteinase-2, with differential patterns observed across uterine structures.

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The study examined immunohistochemical expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in uterine tissue from 32 reproductive-age cows, grouped by adenomyosis degree (superficial vs deep) and sampled during the follicular phase. Using monoclonal antibodies, staining intensity was evaluated across multiple uterine compartments, including luminal epithelium, ectopic/dystopic endometrial tissue, endometrial-myometrial border, myometrium, and myometrial vessels. MMP-2 expression was higher in deep adenomyosis across several structures, and MMP-2 generally exceeded TIMP-2 in deep cases, whereas TIMP-2 exceeded MMP-2 in normal specimens and in superficial adenomyosis during the follicular phase. This paper is centrally about endometriosis and/or adenomyosis — it directly investigates adenomyosis in bovine uteri by characterizing differential MMP-2 and TIMP-2 expression patterns.

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Abstract

The present investigation was intended to show a different immunohistochemical profile of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 in bovine uteri with adenomyosis during follicular phase. Uterine samples of 32 cows in reproductive age were taken from the medial third of one of the uterine horns and grouped according to the adenomyosis degree (superficial and deep). Tissue sections (4 μm) were incubated overnight at 4°C with monoclonal antibody for matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2. Staining intensities were evaluated in the luminal epithelium, ectopic and dystopic endometrial tissue (stroma, capillaries and glands), endometrial-myometrial border, myometrium, myometrial vessels (middle tunic and endothelium). The matrix metalloproteinase-2 expression was higher for deep adenomyosis samples, showing a differential mean reactivity in superficial endometrium, myometrial vessels, myometrium adjacent to adenomyotic focus and endometrial-myometrial border (P < 0.05). Moreover, matrix metalloproteinase-2 expression was higher in deep adenomyosis samples than that of Tissue inhibitor metalloproteinase-2 in almost all uterine structures analyzed (except for the endometrial and myometrial vessels and endometrial-myometrial border). The opposite was observed in the follicular phase, for both normal specimens and with superficial adenomyosis, where Tissue inhibitor metalloproteinase-2 expression was higher than that of matrix metalloproteinase-2. In conclusion, a differential pattern of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 was observed in cow uteri with adenomyosis.
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Abstract

The present investigation was intended to show a different immunohistochemical profile of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 in bovine uteri with adenomyosis during follicular phase. Uterine samples of 32 cows in reproductive age were taken from the medial third of one of the uterine horns and grouped according to the adenomyosis degree (superficial and deep). Tissue sections (4 μm) were incubated overnight at 4°C with monoclonal antibody for matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2. Staining intensities were evaluated in the luminal epithelium, ectopic and dystopic endometrial tissue (stroma, capillaries and glands), endometrial-myometrial border, myometrium, myometrial vessels (middle tunic and endothelium). The matrix metalloproteinase-2 expression was higher for deep adenomyosis samples, showing a differential mean reactivity in superficial endometrium, myometrial vessels, myometrium adjacent to adenomyotic focus and endometrial-myometrial border (P < 0.05). Moreover, matrix metalloproteinase-2 expression was higher in deep adenomyosis samples than that of Tissue inhibitor metalloproteinase-2 in almost all uterine structures analyzed (except for the endometrial and myometrial vessels and endometrial-myometrial border). The opposite was observed in the follicular phase, for both normal specimens and with superficial adenomyosis, where Tissue inhibitor metalloproteinase-2 expression was higher than that of matrix metalloproteinase-2. In conclusion, a differential pattern of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 was observed in cow uteri with adenomyosis. Similar content being viewed by others

References

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Acknowledgements

The authors are indebted to the technical personnel of the Morphology and Pathological Anatomy Department, UENF, to the State Slaughterhouses of Campos dos Goytacazes, State of Rio de Janeiro, Brazil, to UENF, FAPERJ, CAPES and CNPq for funding this research. Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Moreira, L., de Carvalho, E.C.Q. & Caldas-Bussiere, M.C. Differential immunohistochemical expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in cow uteri with adenomyosis during follicular phase. Vet Res Commun 35, 261–269 (2011). https://doi.org/10.1007/s11259-011-9470-1 Accepted: Published: Issue date: DOI: https://doi.org/10.1007/s11259-011-9470-1

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Condition tags

endometriosisadenomyosis

MeSH descriptors

Cattle Diseases Endometriosis Endometrium Matrix Metalloproteinase 2 Myometrium Tissue Inhibitor of Metalloproteinase-2 Animals Cattle Cattle Diseases Cattle Diseases Cattle Diseases Endometriosis Endometriosis Endometriosis Endometriosis Endometrium Endometrium Female Follicular Phase Immunohistochemistry

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