Knockdown of E-cadherin expression of endometrial epithelial cells may activate Wnt/β-catenin pathway in vitro

Purpose E-cadherin, a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelial cell, plays an essential role in metastasis. It has been postulated that E-cadherin downregulation is a crucial mechanism in the pathogenesis of endometriosis. To evaluate the effect on the cell behavior after knockdown of E-cadherin gene (CDH1) in cultured human endometrial epithelial cells (EECs) isolated from normal endometrium.

EECs were isolated from the endometrial tissues of fertile woman who underwent total hysterectomy due to cervical intraepithelial neoplasia III. CDH1 expression was knocked down by small hairpin RNA. The EECs transfected with empty vector served as control. Transwell assay was used to test EECs migration or invasion. qRT-PCR and western blot were used to detect mRNA and protein levels.

The results showed that knockdown of E-cadherin expression can increase cell migration and invasion, and up-regulate mRNA and protein levels of β-catenin, cyclinD1, and c-myc.

Down-regulation of E-cadherin expression may activate the Wnt/β-catenin pathway in endometrial cells, which may together participate in the occurrence of endometriosis. Similar content being viewed by others

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YL: Protocol development and manuscript writing. XZ: Performed the experiments and manuscript writing. RZ: Data analysis. NW: Data analysis. Corresponding author Ethics declarations Ethics approval This study was approved by Fourth Hospital’s Ethics Committee, Hebei Medical University. Informed consent Informed consent was obtained from all recruited subjects. Conflict of interest The authors declare that there is no conflict of interest. Funding This work was supported by a grant from the National Natural Science Foundation of China (no. 81270673). Electronic supplementary material Below is the link to the electronic supplementary material. Rights and permissions About this article Cite this article Zhu, X., Li, Y., Zhou, R. et al. Knockdown of E-cadherin expression of endometrial epithelial cells may activate Wnt/β-catenin pathway in vitro. Arch Gynecol Obstet 297, 117–123 (2018). https://doi.org/10.1007/s00404-017-4560-0 Received: Accepted: Published: Issue date: DOI: https://doi.org/10.1007/s00404-017-4560-0