Direct Effect of Danazol on the DNA Synthesis and Ultrastructure of Human Cultured Endometrial Stromal Cells

Makoto Taguchi, Toshiro Kubota, Takeshi Asô
In: Gynecologic and Obstetric Investigation · 2010 · vol. 39(3) , pp. 192–196 · doi:10.1159/000292407 · PMID:7789916 · W2023296993
article OA: closed CC0 ⤵ 7 in-corpus citations
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Danazol dose-dependently inhibited DNA synthesis in human endometrial stromal cells, likely via androgen receptors, and at high concentrations, induced cell death with organelle destruction.

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Abstract

The direct effect of danazol on the DNA synthesis and ultrastructure of human cultured endometrial stromal cells was studied. The analysis of the [3H]thymidine incorporation in the endometrial stromal cells cultured for 10 days indicated that the DNA synthesis was inhibited in a dose-dependent manner by danazol and testosterone, but not by medroxyprogesterone. The effect of 10(-7) M of danazol was blocked by cyproterone acetate, but RU-486 did not show any influence. The observation of the ultrastructure of the cultured cells revealed that 10(-7) M of danazol increased cytoplasmic lysosomes; however, 10(-5) M of danazol destroyed cell organelles and cytoskeleton resulted from the cell death. These results indicated that the biological effects of danazol were mainly mediated by androgen receptors, and 10(-5) M of danazol exhibited the toxic effect on the endometrial stromal cells.

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