Non-thermal plasma prevents progression of endometriosis in mice

Chiharu Ishida, Ishida C, Masahiko Mori, Mori M, Kae Nakamura, Nakamura K, Tanaka H, Hiromasa Tanaka, Mizuno M, Masaaki Mizuno, Hori M, Masaru Hori, Akira Iwase, Iwase A, Kikkawa F, Fumitaka Kikkawa, Shinya Toyokuni
Free radical research · 2016 · vol. 50(10) , pp. 1131–1139 · doi:10.1080/10715762.2016.1211273 · PMID:27396236 · W2469783154
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⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

Non-thermal plasma treatment of surgically excised uterine tissue in mice significantly reduced endometriotic cyst surface area and epithelial proliferation compared to controls.

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⚙ AI-generated deep summary by claude@2026-07, 2026-07-09 · read from full text ⓘ

This preclinical study evaluated non-thermal plasma (NTP) as a surgical treatment to prevent endometriosis progression in a BALB/c mouse model. Using estradiol-supplemented transplantation of excised uteri, the researchers exposed each endometriotic lesion to NTP after laparotomy and compared outcomes with helium-exposed controls; in vitro, epithelial cells were most sensitive to NTP-activated medium while ectopic endometrial stromal cells were most resistant. Immunohistochemistry showed that immediately after NTP exposure, epithelial cells had significantly higher nuclear 8-hydroxy-2′-deoxyguanosine staining than stromal cells, and by four weeks total endometriotic cyst surface area was significantly smaller with reduced epithelial proliferative activity, while lesion number did not change. The paper’s limitation is its reliance on a mouse model and lesion-level outcomes rather than demonstration of complete eradication. This paper is centrally about endometriosis — it tests non-thermal plasma to prevent progression and recurrence of endometriosis in mice.

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Abstract

Endometriosis is observed in ∼10% of reproductive age women. Ovarian endometriosis not only causes dysmenorrhea but also causes infertility and a high risk of adenocarcinoma. Due to its scattered nature, complete surgical resection is difficult. Endometriosis consists of glandular and stromal cells. Previously, we showed that endometrial stromal cells (ESCs) play a role in the protection against pathologic events caused by monthly repeated hemorrhage. Here, we undertook a preclinical study of non-thermal plasma (NTP) as a surgical treatment of endometriosis. Epithelial cells were most sensitive to NTP-activated medium in vitro, whereas ectopic ESCs were most resistant. We then transplanted excised uteruses into BALB/c mice from donors of the same strain with estradiol supplementation. Four weeks after the transplantation, we exposed NTP to each endometriotic lesion after laparotomy. Immunohistochemical analysis revealed that immediately after NTP exposure, epithelial cells exhibited significantly higher levels of nuclear immunostaining for 8-hydroxy-2'-deoxyguanosine than did stromal cells. Four weeks after NTP exposure, the total surface area consisting of endometriotic cysts was significantly smaller with less epithelial proliferative activity than the helium-exposed control, whereas the number of endometriotic lesions had not changed. Therefore, NTP exposure may be useful to prevent the progression and recurrence of endometriosis.
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WEKO3 アイテム Non-thermal plasma prevents progression of endometriosis in mice http://hdl.handle.net/2237/25581 http://hdl.handle.net/2237/25581a04e8e4e-9583-4aea-bbb6-440f13bee136 | 名前 / ファイル | ライセンス | アクション | |---|---|---| | Ishida_Plasma_endometriosis_052516_FFB.pdf ファイル公開：2017/08/08 (4.9 MB) | | | アイテムタイプ | 学術雑誌論文 / Journal Article(1) | ||||| |---|---|---|---|---|---|---| | 公開日 | 2017-02-16 | ||||| | タイトル | |||||| | タイトル | Non-thermal plasma prevents progression of endometriosis in mice | ||||| | 言語 | en | ||||| | 著者 | Ishida, Chiharu × Ishida, Chiharu× Mori, Masahiko× Nakamura, Kae× Tanaka, Hiromasa× Mizuno, Masaaki× Hori, Masaru× Iwase, Akira× Kikkawa, Fumitaka× Toyokuni, Shinya | ||||| | アクセス権 | |||||| | アクセス権 | open access | ||||| | アクセス権URI | http://purl.org/coar/access_right/c_abf2 | ||||| | 権利 | |||||| | 権利情報 | This is an Accepted Manuscript of an article published by Taylor & Francis Group in Free Radical Research on 08/08/2016, available online: http://www.tandfonline.com/10.1080/10715762.2016.1211273. | ||||| | 言語 | en | ||||| | キーワード | |||||| | 主題Scheme | Other | ||||| | 主題 | Endometriosis | ||||| | キーワード | |||||| | 主題Scheme | Other | ||||| | 主題 | mouse model | ||||| | キーワード | |||||| | 主題Scheme | Other | ||||| | 主題 | non-thermal plasma | ||||| | キーワード | |||||| | 主題Scheme | Other | ||||| | 主題 | oxidative stress | ||||| | 抄録 | |||||| | 内容記述タイプ | Abstract | ||||| | 内容記述 | Endometriosis is observed in ∼10% of reproductive age women. Ovarian endometriosis not only causes dysmenorrhea but also causes infertility and a high risk of adenocarcinoma. Due to its scattered nature, complete surgical resection is difficult. Endometriosis consists of glandular and stromal cells. Previously, we showed that endometrial stromal cells (ESCs) play a role in the protection against pathologic events caused by monthly repeated hemorrhage. Here, we undertook a preclinical study of non-thermal plasma (NTP) as a surgical treatment of endometriosis. Epithelial cells were most sensitive to NTP-activated medium in vitro, whereas ectopic ESCs were most resistant. We then transplanted excised uteruses into BALB/c mice from donors of the same strain with estradiol supplementation. Four weeks after the transplantation, we exposed NTP to each endometriotic lesion after laparotomy. Immunohistochemical analysis revealed that immediately after NTP exposure, epithelial cells exhibited significantly higher levels of nuclear immunostaining for 8-hydroxy-2′-deoxyguanosine than did stromal cells. Four weeks after NTP exposure, the total surface area consisting of endometriotic cysts was significantly smaller with less epithelial proliferative activity than the helium-exposed control, whereas the number of endometriotic lesions had not changed. Therefore, NTP exposure may be useful to prevent the progression and recurrence of endometriosis. | ||||| | 言語 | en | ||||| | 出版者 | |||||| | 出版者 | Taylor & Francis | ||||| | 言語 | en | ||||| | 言語 | |||||| | 言語 | eng | ||||| | 資源タイプ | |||||| | 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | ||||| | タイプ | journal article | ||||| | 出版タイプ | |||||| | 出版タイプ | AM | ||||| | 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | ||||| | DOI | |||||| | 関連タイプ | isVersionOf | ||||| | 識別子タイプ | DOI | ||||| | 関連識別子 | https://doi.org/10.1080/10715762.2016.1211273 | ||||| | ISSN | |||||| | 収録物識別子タイプ | PISSN | ||||| | 収録物識別子 | 1071-5762 | ||||| | 書誌情報 | en : Free Radical Research 巻 50, 号 10, p. 1131-1139, 発行日 2016-08-08 | ||||| | 著者版フラグ | |||||| | 値 | author | ||||| | URI | |||||| | 識別子 | http://doi.org/10.1080/10715762.2016.1211273 | ||||| | 識別子タイプ | DOI | ||||| | URI | |||||| | 識別子 | http://hdl.handle.net/2237/25581 | ||||| | 識別子タイプ | HDL |

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Condition tags

endometriosisdysmenorrheainfertility

MeSH descriptors

Endometriosis Plasma Gases Animals Cell Proliferation Disease Models, Animal Disease Progression Endometriosis Female Mice Mice, Inbred BALB C Plasma Gases

Citation neighborhood

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References (38)

Cited by (3)

SciLite annotations

organisms 3
mus sp. noordeloos 2009062 enterobacteriaceae bacterium c/sb65
chemicals 4
estradiol hydroxyl 5'-d[ctgccttcag]-3' helium

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