Peer Review #2 of "TET1 may contribute to hypoxia-induced epithelial to mesenchymal transition of endometrial epithelial cells in endometriosis (v0.1)"

2020 · doi:10.7287/peerj.9950v0.1/reviews/2 · W4255807458
Lab / animal OA: gold CC0
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TET1 demethylase, upregulated by HIF-2α under hypoxia, contributes to epithelial-mesenchymal transition in endometrial epithelial cells, potentially playing a role in endometriosis.

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Abstract

Background.Endometriosis (EMs) is a non-malignant gynecological disease, whose pathogenesis remains to be clarified.Recent studies have found that hypoxia induces epithelial-mesenchymal transition (EMT) as well as epigenetic modification in EMs.However, the relationship between EMT and demethylation modification under hypoxia status in EMs remains unknown.Methods.The expression of N-cadherin, E-cadherin and TET1 in normal endometria, eutopic endometria and ovarian endometriomas was assessed by immunohistochemistry and immunofluorescence double staining.5-hmC was detected by fluorescence-based ELISA kit using a specific 5-hmC antibody.Overexpression and inhibition of TET1 or hypoxia-inducible factor 2α (HIF-2α) were performed by plasmid and siRNA transfection.The expression of HIF-2α, TET1 and EMT markers in Ishikawa (ISK) cells (widely used as endometrial epithelial cells) was evaluated by western blotting.The interaction of HIF-2α and TET1 was analyzed by chromatin immunoprecipitation.Results.Demethylation enzyme TET1 (ten-eleven translocation1) was elevated in glandular epithelium of ovarian endometrioma, along with the activation of EMT (increased expression of N-cadherin, and decreased expression of E-cadherin) and global increase of epigenetic modification marker 5-hmC(5hydroxymethylcytosine).Besides, endometriosis lesions had more TET1 and N-cadherin co-localized cells.Further study showed that ISK cells exhibited enhanced EMT, and increased expression of TET1 and HIF-2α under hypoxic condition.Hypoxia-induced EMT was partly regulated by TET1 and HIF-2α.HIF-2α inhibition mitigated TET1 expression changes provoked by hypoxia.Conclusions.Hypoxia induces the expression of TET1 regulated by HIF-2α, thus may promote EMT in endometriosis.

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Condition tags

endometriosisendometrioma

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (51)

Source provenance

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