Cirsilineol inhibits the proliferation and migration of endometriotic cells

Xumeng Gu, Tianchan Zhang, Tongtong Liu, Huifang Cong, Wenpeng Wu
In: Tropical Journal of Pharmaceutical Research · 2024 · vol. 22(12) · doi:10.4314/tjpr.v22i12.2 · W4390976160
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Cirsilineol inhibited human endometriotic cell proliferation and migration by arresting the cell cycle at G2/M and downregulating p65 phosphorylation, indicating a dose-dependent effect via NF-κB pathway regulation.

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⚙ AI-generated deep summary by qwen3.7-flash, 2026-09-03 · read from full text ⓘ

This study investigated the effects of cirsilineol on human endometriotic epithelial 12Z cells to determine its potential as a therapeutic agent. Researchers treated the cells with varying concentrations of the compound and assessed outcomes through proliferation, migration, invasion, and cell cycle assays alongside Western blot analysis of NF-κB pathway components. The results demonstrated that doses of 10 and 20 μM significantly reduced proliferation by inducing G2/M phase arrest while suppressing migration and invasion via downregulation of p65 phosphorylation and modulation of IκBα expression. This paper is centrally about endometriosis — specifically the in vitro inhibition of endometriotic cell growth and spread using cirsilineol targeting the NF-κB signaling pathway.

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Abstract

Purpose: To determine the effect of cirsilineol on the proliferation and migration of endometriotic cells. Methods: Human endometriotic epithelial cells 12Z were treated with 5, 10 and 20 μM cirsilineol, respectively. Cell proliferation was evaluated using BrdU incorporation assay whereas wound healing assay was used to investigate cell migration, and cell invasion was assessed by Transwell assay. Cell cycle was investigated using flow cytometry while protein expression and phosphorylation levels of p65 and IκBα were evaluated by Western blot. Results: Treatment with cirsilineol at either 10 μM or 20 μM resulted in a significant reduction in cell proliferation and cell cycle arrest at the G2/M phase (p < 0.05). Moreover, cirsilineol weakened cell migration and invasion in 12Z cells, but did not alter the expression of p65. However, it significantly downregulated the phosphorylation of p65 and increased the expression of IκBα, while attenuating the phosphorylation level of IκBα (p < 0.05). Furthermore, 5 μM cirsilineol did not show any significant effects on these cellular activities, suggesting the activity of cirsilineol in 12Z cells is dose-dependent. Conclusion: Cirsilineol inhibits cell proliferation, induces cell cycle arrest, suppresses migration and invasion of endometriotic epithelial cells by regulating NF-κB pathway. Thus, cirsilineol might be a potential therapeutic candidate for endometriosis treatmen
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Abstract

Methods: Human endometriotic epithelial cells 12Z were treated with 5, 10 and 20 μM cirsilineol, respectively. Cell proliferation was evaluated using BrdU incorporation assay whereas wound healing assay was used to investigate cell migration, and cell invasion was assessed by Transwell assay. Cell cycle was investigated using flow cytometry while protein expression and phosphorylation levels of p65 and IκBα were evaluated by Western blot.

Results

Treatment with cirsilineol at either 10 μM or 20 μM resulted in a significant reduction in cell proliferation and cell cycle arrest at the G2/M phase (p < 0.05). Moreover, cirsilineol weakened cell migration and invasion in 12Z cells, but did not alter the expression of p65. However, it significantly downregulated the phosphorylation of p65 and increased the expression of IκBα, while attenuating the phosphorylation level of IκBα (p < 0.05). Furthermore, 5 μM cirsilineol did not show any significant effects on these cellular activities, suggesting the activity of cirsilineol in 12Z cells is dose-dependent.

Conclusion

Cirsilineol inhibits cell proliferation, induces cell cycle arrest, suppresses migration and invasion of endometriotic epithelial cells by regulating NF-κB pathway. Thus, cirsilineol might be a potential therapeutic candidate for endometriosis treatmen

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endometriosis

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