Trichrome Mallory's stain may indicate differential rates of RNA synthesis in eutopic and ectopic endometrium.

Maria Wołuń-Cholewa, Krzysztof Szymanowski, Mirosław Andrusiewicz, Anna Szczerba, Jerzy B Warchoł
Folia histochemica et cytobiologica · 2010 · vol. 48(1) , pp. 148–52 · doi:10.2478/v10042-008-0106-4 · PMID:20529831 · W2169741083
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Mallory's stain revealed distinct nuclear staining patterns in eutopic versus ectopic endometrium, suggesting differential RNA synthesis rates, with aniline blue affinity in normal/hyperplastic endometrium disappearing after RNase digestion.

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The paper investigated whether Mallory’s triple histochemical staining reflects differences in nuclear staining patterns and inferred RNA synthesis between eutopic and ectopic endometrium, with additional comparisons to endometrial hyperplasia and adenocarcinoma. Using Mallory’s method, the authors reported that stromal nuclei in normal and hyperplastic endometrium showed increased affinity to aniline blue, an effect that disappeared after RNase digestion, consistent with RNA-associated staining, whereas in endometriosis nuclei showed affinity to orange G regardless of cell type; similar orange G findings were noted in adenocarcinoma. Ultrastructural analyses found euchromatic, low electron density stromal nuclei in normal endometrium but heterochromatic nuclei in endometriosis both in stroma and within glands. This paper is centrally about endometriosis — it examines how Mallory’s stain distinguishes eutopic versus ectopic endometrium and interprets staining as differential RNA synthesis.

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Abstract

Mallory's triple staining is a histochemical technique used mainly for analysing connective tissues and glands and other tissues. We have described the differences in the nuclear staining between eutopic and ectopic endometrium as well as endometrial hyperplasia and adenocarcinoma using the Mallory's method. The ultrastructural differences between eutopic and ectopic endometrium have been detected. In normal and hyperplastic endometrium the presence of stromal cell nuclei with an increased affinity to aniline blue has been observed. The affinity has disappeared after digestion of tissues with RNase. In cases of endometriosis, independently of cell types, the nuclei have shown affinity to orange G. Similar effects in adenocarcinoma have been noted. The ultrastructural studies have shown that in normal endometrium the stroma contained cells with euchromatic and low electron density cell nuclei. In endometriosis heterochromatic cell nuclei present both in the stroma and within glands have been detected. The results indicate that the Mallory's technique may be a useful tool for recognizing the differences between eutopic and ectopic endometrium. The affinity for aniline blue in normal and hyperplastic endometrium occurs most likely due to increased RNA synthesis. Based on Mallory's staining a similarity between hyperplasia and unchanged endometrium in contrast to similar results of the staining obtained in cases of adenocarcinoma and endometriosis may be suggested.
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Vol 48, No 1 (2010) Original paper Published online: 2010-06-10 Trichrome Mallory's stain may indicate differential rates of RNA synthesis in eutopic and ectopic endometrium. DOI: 10.2478/v10042-008-0106-4 Folia Histochem Cytobiol 2010;48(1):148-152. Abstract Mallory's triple staining is a histochemical technique used mainly for analysing connective tissues and glands and other tissues. We have described the differences in the nuclear staining between eutopic and ectopic endometrium as well as endometrial hyperplasia and adenocarcinoma using the Mallory's method. The ultrastructural differences between eutopic and ectopic endometrium have been detected. In normal and hyperplastic endometrium the presence of stromal cell nuclei with an increased affinity to aniline blue has been observed. The affinity has disappeared after digestion of tissues with RNase. In cases of endometriosis, independently of cell types, the nuclei have shown affinity to orange G. Similar effects in adenocarcinoma have been noted. The ultrastructural studies have shown that in normal endometrium the stroma contained cells with euchromatic and low electron density cell nuclei. In endometriosis heterochromatic cell nuclei present both in the stroma and within glands have been detected. The results indicate that the Mallory's technique may be a useful tool for recognizing the differences between eutopic and ectopic endometrium. The affinity for aniline blue in normal and hyperplastic endometrium occurs most likely due to increased RNA synthesis. Based on Mallory's staining a similarity between hyperplasia and unchanged endometrium in contrast to similar results of the staining obtained in cases of adenocarcinoma and endometriosis may be suggested.

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Condition tags

endometriosis

MeSH descriptors

Azo Compounds Choristoma Endometrium Eosine Yellowish-(YS) Methyl Green RNA Staining and Labeling Adult Azo Compounds Cell Nucleus Cell Nucleus Cell Nucleus Choristoma Choristoma Endometrium Endometrium Endometrium Eosine Yellowish-(YS) Female Humans

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