Expression of membrane progesterone receptors in eutopic and ectopic endometrium of women with endometriosis

Edgar Ricardo Vázquez-Martínez, Claudia Bello‐Alvarez, Ana Lorena Hermenegildo-Molina, Juan Mario Solis-Paredes, Sandra Parra-Hernández, Oliver Cruz-Orozco, José Roberto Silvestri-Tomassoni, Luis Fernando Escobar-Ponce, Luis A. Hernández-López, Christian Reyes-Mayoral, Andrea Olguín-Ortega, Brenda Sánchez-Ramírez, Mauricio Osorio‐Caballero, Elizabeth García-Gómez, Guadalupe Estrada‐Gutiérrez, Marco Cerbón, Ignacio Camacho‐Arroyo
In: Research Square · 2020 · doi:10.21203/rs.3.rs-16694/v1 · W4246620198
preprint OA: green CC0 ⤵ 1 in-corpus citation
🔓 Open OA copy View on OpenAlex View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study found decreased expression and protein content of membrane progesterone receptors (mPRs) in eutopic and ectopic endometrium of women with endometriosis, potentially contributing to progesterone resistance.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-06, 2026-06-08 ⓘ

This study assessed mRNA expression and protein content of membrane progesterone receptors from the PAQR family (PAQR7/mPRα, PAQR8/mPRβ, PAQR5/mPRγ, and PAQR6/mPRδ) in endometrium from women with ovarian endometriosis and in control endometrium. Using RT-qPCR and Western blot on samples from eutopic endometrium of 17 patients and 17 controls, plus ectopic lesion tissues from 9 patients, the authors found reduced PAQR7 and PAQR5 mRNA in both eutopic and ectopic endometrium, and reduced PAQR8 and PAQR6 mRNA specifically in eutopic endometrium; mPRα and mPRβ protein content were decreased in ectopic endometrium. A key caveat is the observational design with relatively small and phase-influenced sampling (many samples in the proliferative phase, and ectopic tissues split across lesion types). This paper is centrally about endometriosis — specifically, it analyzes membrane progesterone receptor (PAQR/mPR) expression in eutopic versus ectopic endometrium in women with endometriosis.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Abstract Background: Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by non-genomic mechanisms via membrane progesterone receptors that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of PAQR family members in the eutopic and ectopic endometrium of women with endometriosis. Methods : Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 ( mPRα ) , PAQR8 , (mPRβ) PAQR5 ( mPRγ ) , and PAQR6 ( mPRδ ) at mRNA and protein levels was evaluated by RT-qPCR and western blot. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey's multiple comparisons test with a confidence interval of 95%. Results : The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Conclusions : Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.

My notes (saved in your browser only)

Condition tags

endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (29)

Cited by (1)

Source provenance

europepmc
last seen: 2026-06-12T06:25:08.868844+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK