Special Issue "Flow Cytometry: Applications and Challenges"

Falda A
International journal of molecular sciences · 2026 · vol. 27(17) · doi:10.3390/ijms27177899 · PMID:42737796 · PMC13566490
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This special issue highlights the continued relevance of flow cytometry across various fields, addressing its ongoing applications and associated challenges despite the availability of molecular tests and imaging techniques.

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This special issue introduction highlights a collection of studies focusing on the applications and technical challenges of flow cytometry across various biological and medical domains. The included papers address diverse topics such as detecting clonal precursors in acute leukemias, analyzing T-cell populations, assessing hematopoietic stem cells, and monitoring microbial dynamics during fermentation processes. Several contributions also examine specific methodological improvements, including spectral flow cytometry, antibody selection tools, and basophil activation tests for allergy diagnostics. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Despite the use of molecular tests and imaging techniques, flow cytometry continues to capture attention in various fields today [...].
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I would like to thank all the contributing authors for participating in this Special Issue on the applications and challenges of flow cytometry.

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Álvarez Flores, M.B.; Sopeña Corvinos, M.; Guillén Santos, R.; Cava Valenciano, F. Fluorescent Aerolysin (FLAER) Binding Is Abnormally Low in the Clonal Precursors of Acute Leukemias, with Binding Particularly Low or Absent in Acute Promyelocytic Leukemia. Int. J. Mol. Sci. 2024 , 25 , 11898. https://doi.org/10.3390/ijms252211898 . Buček, S.; Brožič, A.; Miceska, S.; Gašljević, G.; Kloboves Prevodnik, V. Clustering Algorithm-Driven Detection of TRBC1-Restricted Clonal T-Cell Populations Produces Better Results than Manual Gating Analysis. Int. J. Mol. Sci. 2024 , 26 , 170. https://doi.org/10.3390/ijms26010170 . Semchenkova, A.; Mikhailova, E.; Demina, I.; Roumiantseva, J.; Karachunskiy, A.; Novichkova, G.; Popov, A. Analysis of Antigen Expression in T-Cell Acute Lymphoblastic Leukemia by Multicolor Flow Cytometry: Implications for the Detection of Measurable Residual Disease. Int. J. Mol. Sci. 2025 , 26 , 2002. https://doi.org/10.3390/ijms26052002 . Astakhova, E.A.; Gubaeva, A.S.; Naumova, D.A.; Egorova, A.E.; Maznina, A.A.; Rybkina, I.G.; Osmanov, I.M.; Tabakov, D.V.; Mityaeva, O.N.; Volchkov, P.Y. Spectral Flow Cytometry: The Current State and Future of the Technology. Int. J. Mol. Sci. 2025 , 26 , 5911. https://doi.org/10.3390/ijms26125911 . Piasecki, A.J.; Sheehan, H.C.; Tilly, J.L.; Woods, D.C. Flow Cytometric Quantification of Mitochondrial Properties: A High-Throughput Approach for Single Organelle Analysis. Int. J. Mol. Sci. 2025 , 26 , 5481. https://doi.org/10.3390/ijms26125481 . Poncelet, P.; Lecompte, T.; Bauters, A.; Mullier, F. Quantitative Flow Cytometry-Medical Applications with a Focus on Blood Platelets. Int. J. Mol. Sci. 2026 , 27 , 1976. https://doi.org/10.3390/ijms27041976 . Serra, V.; Orrù, V.; Lai, S.; Dei, M.; Marongiu, M.; Piras, M.G.; Virdis, F.; Floris, M.; Delogu, G.; Lodde, V.; et al. FlowCLOc, a New Tool for Selecting the Most Appropriate Antibodies in Flow Cytometry. Int. J. Mol. Sci. 2026 , 27 , 1664. https://doi.org/10.3390/ijms27041664 . Schmachtel, T.; Bonig, H.; Rieger, M.A. FACS-Based Assessment of Human Hematopoietic Stem and Progenitor Cells. Int. J. Mol. Sci. 2025 , 26 , 8381. https://doi.org/10.3390/ijms26178381 . Demeter, S.; Fekete, T.; Scholtz, B.; Veréb, Z.; Kemény, L.; Bácsi, A.; Pázmándi, K. Flow Cytometric Challenges in Plasmacytoid Dendritic Cell (pDC) Identification: Limitation of BDCA-4 (CD304)-Based Gating. Int. J. Mol. Sci. 2025 , 26 , 10979. https://doi.org/10.3390/ijms262210979 . Falda, A. Macrophage Plasticity: Phenotypic and Functional Profiles Across Pathological Microenvironments. Int. J. Mol. Sci. 2026 , 27 , 5333. https://doi.org/10.3390/ijms27125333 . Groffmann, J.; Hoppe, I.; Ahmed, W.A.; Bast, D.; Brinster, S.; Altintas, S.; Schusta, F.; Weigt, K.; Worm, M.; Beyer, K.; et al. Improved bin-based basophil activation test facilitates comparison of wheat allergy and tolerance in children and adults. Int. J. Mol. Sci. 2026 , 27 , 1620. https://doi.org/10.3390/ijms27041620 . Sizzano, F.; Bianconi, V.; Dorsaz, E.; Boilley, A.; Berthoud, H.; Bridy, N.; Amiet, L.; Bourdin, G. Flow Cytometry-Based Monitoring of Microbial Dynamics During Grape Must Fermentation Under Different Inoculation Strategies. Int. J. Mol. Sci. 2026 , 27 , 1414. https://doi.org/10.3390/ijms27031414 .

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The author declares no conflicts of interest.

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Flow Cytometry Flow Cytometry Humans

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