{"paper_id":"c91bb738-8be1-4602-9469-0b18f7e45307","body_text":"I would like to thank all the contributing authors for participating in this Special Issue on the applications and challenges of flow cytometry.\n\nThe author declares no conflicts of interest.\n\nÁlvarez Flores, M.B.; Sopeña Corvinos, M.; Guillén Santos, R.; Cava Valenciano, F. Fluorescent Aerolysin (FLAER) Binding Is Abnormally Low in the Clonal Precursors of Acute Leukemias, with Binding Particularly Low or Absent in Acute Promyelocytic Leukemia.  Int. J. Mol. Sci. \n 2024 ,  25 , 11898.  https://doi.org/10.3390/ijms252211898 .\nBuček, S.; Brožič, A.; Miceska, S.; Gašljević, G.; Kloboves Prevodnik, V. Clustering Algorithm-Driven Detection of TRBC1-Restricted Clonal T-Cell Populations Produces Better Results than Manual Gating Analysis.  Int. J. Mol. Sci. \n 2024 ,  26 , 170.  https://doi.org/10.3390/ijms26010170 .\nSemchenkova, A.; Mikhailova, E.; Demina, I.; Roumiantseva, J.; Karachunskiy, A.; Novichkova, G.; Popov, A. Analysis of Antigen Expression in T-Cell Acute Lymphoblastic Leukemia by Multicolor Flow Cytometry: Implications for the Detection of Measurable Residual Disease.  Int. J. Mol. Sci. \n 2025 ,  26 , 2002.  https://doi.org/10.3390/ijms26052002 .\nAstakhova, E.A.; Gubaeva, A.S.; Naumova, D.A.; Egorova, A.E.; Maznina, A.A.; Rybkina, I.G.; Osmanov, I.M.; Tabakov, D.V.; Mityaeva, O.N.; Volchkov, P.Y. Spectral Flow Cytometry: The Current State and Future of the Technology.  Int. J. Mol. Sci. \n 2025 ,  26 , 5911.  https://doi.org/10.3390/ijms26125911 .\nPiasecki, A.J.; Sheehan, H.C.; Tilly, J.L.; Woods, D.C. Flow Cytometric Quantification of Mitochondrial Properties: A High-Throughput Approach for Single Organelle Analysis.  Int. J. Mol. Sci. \n 2025 ,  26 , 5481.  https://doi.org/10.3390/ijms26125481 .\nPoncelet, P.; Lecompte, T.; Bauters, A.; Mullier, F. Quantitative Flow Cytometry-Medical Applications with a Focus on Blood Platelets.  Int. J. Mol. Sci. \n 2026 ,  27 , 1976.  https://doi.org/10.3390/ijms27041976 .\nSerra, V.; Orrù, V.; Lai, S.; Dei, M.; Marongiu, M.; Piras, M.G.; Virdis, F.; Floris, M.; Delogu, G.; Lodde, V.; et al. FlowCLOc, a New Tool for Selecting the Most Appropriate Antibodies in Flow Cytometry.  Int. J. Mol. Sci. \n 2026 ,  27 , 1664.  https://doi.org/10.3390/ijms27041664 .\nSchmachtel, T.; Bonig, H.; Rieger, M.A. FACS-Based Assessment of Human Hematopoietic Stem and Progenitor Cells.  Int. J. Mol. Sci. \n 2025 ,  26 , 8381.  https://doi.org/10.3390/ijms26178381 .\nDemeter, S.; Fekete, T.; Scholtz, B.; Veréb, Z.; Kemény, L.; Bácsi, A.; Pázmándi, K. Flow Cytometric Challenges in Plasmacytoid Dendritic Cell (pDC) Identification: Limitation of BDCA-4 (CD304)-Based Gating.  Int. J. Mol. Sci. \n 2025 ,  26 , 10979.  https://doi.org/10.3390/ijms262210979 .\nFalda, A. Macrophage Plasticity: Phenotypic and Functional Profiles Across Pathological Microenvironments.  Int. J. Mol. Sci. \n 2026 ,  27 , 5333.  https://doi.org/10.3390/ijms27125333 .\nGroffmann, J.; Hoppe, I.; Ahmed, W.A.; Bast, D.; Brinster, S.; Altintas, S.; Schusta, F.; Weigt, K.; Worm, M.; Beyer, K.; et al. Improved bin-based basophil activation test facilitates comparison of wheat allergy and tolerance in children and adults.  Int. J. Mol. Sci. \n 2026 ,  27 , 1620.  https://doi.org/10.3390/ijms27041620 .\nSizzano, F.; Bianconi, V.; Dorsaz, E.; Boilley, A.; Berthoud, H.; Bridy, N.; Amiet, L.; Bourdin, G. Flow Cytometry-Based Monitoring of Microbial Dynamics During Grape Must Fermentation Under Different Inoculation Strategies.  Int. J. Mol. Sci. \n 2026 ,  27 , 1414.  https://doi.org/10.3390/ijms27031414 .\n\nDisclaimer/Publisher’s Note:  The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.","source_license":"CC-BY-4.0","license_restricted":false}