Circular RNAs in endometriosis analyzed through RNA sequencing and bioinformatics for expression profile

Yan Yue, Jing Qian, Dan-Dan Gui, Dandan Gui, Bin Lu, Bin Lü
PeerJ · 2025 · vol. 13 , pp. e19298 · doi:10.7717/peerj.19298 · PMID:40256743 · PMC12009022 · W4409510239
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

This study analyzed circRNA expression profiles in endometriosis patients versus controls using RNA sequencing, identifying differential expression of 679 circRNAs and proposing a potential pathogenic mechanism involving hsa_circ_000005 and hsa_circ_000011.

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Abstract

BACKGROUND: Endometriosis severely affects women's physical and mental health; it is particularly important to find targets for the treatment and diagnosis of endometriosis. METHOD: This research aimed to investigate the circRNA expression pattern in endometriosis, a type of non-coding RNA that can modulate parental gene expression by acting as miRNA sponges. Through high-throughput sequencing, we analyzed the circRNA expression profile in endometriosis patients in comparison to individuals without the condition. RESULTS: We detected 371 circular RNAs (circRNAs) showing increased expression and 308 circRNAs displaying decreased expression levels. To validate these findings, we employed quantitative real-time PCR (qRT-PCR) to confirm the expression of the top three differential expressed circRNAs listed in circBase. We inferred potential roles of these differentially expressed circRNAs in endometriosis development by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Moreover, by examining the circRNA-microRNA-target gene network, we uncovered a plausible mechanism. Specifically, interactions involving the markedly upregulated hsa_circ_000005 and significantly downregulated hsa_circ_000011 with miR-5787 may influence downstream targets, potentially contributing to the pathogenesis of endometriosis. Our study offers a foundational and crucial circRNA expression profile within the framework of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (35)

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