Erratum to: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis

In: Reproductive Biology and Endocrinology · 2011 · vol. 9(1) · doi:10.1186/1477-7827-9-44 · PMC3079635 · W4231514087
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This erratum corrects a previous publication by adding missing data to Table 1 and including (TOF) in the text, along with a revised sentence for the discussion.

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AI-generated deep summary by claude@2026-06, 2026-06-11 · read from full text

This paper is an erratum to a previously published study that used TRIzol treatment of secretory phase endometrium to enable combined proteomic and mRNA microarray analysis of the same samples in women with and without endometriosis, alongside a correction to missing information (in Table 1) and a missing “(TOF)” word in the results/discussion text. It provides an adapted corrected sentence and a revised Table 1 listing representative molecular weights of proteins identified in the related mRNA microarray study, including multiple listed isoforms. The stated limitation is that the original discussion emphasized future work to repeat the study in a larger sample with well defined endometrial samples across menstrual, follicular, and secretory phases to validate reproducibility of SELDI-TOF MS and identify protein peaks. This paper relates to endometriosis because it corrects and clarifies a study specifically conducted in women with and without endometriosis, focused on secretory phase endometrium proteomic and gene expression profiling.

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C O R R E C T I O N Open Access TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis Amelie Fassbender, Peter Simsa, Cleophas M Kyama, Etienne Waelkens, Attila Mihalyi, Christel Meuleman, Olivier Gevaert, Raf Van de Plas, Bart de Moor and Thomas M D ’Hooghe1,2,3,4* Since publication of our article [1], we have realised that we did not include the full data in Table 1 and missed the word (TOF) in the result/discussion section. We have provided here the ada pted sentence and another version of the table, including all the information intended. Results/Discussion “Therefore, we plan to repeat this study in a larger sam- ple size including well defined endometrial samples obtained during menstrual, follicular and secretory phase, to validate the reproducibility of SELDI-TOF MS technology in these samples and to identify the protein* Correspondence: [email protected] 1Leuven University Fertility Centre, Department of Obstetrics & Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium Full list of author information is available at the end of the article Table 1 The representative molecular weights of the proteins identified in the mRNA Microarray study [Thirteen] [2] Protein Mass in Da Osteoglycin (OGN/4969) 33,922 Interleukin-6 signal transducer (IL6ST/3572) isoform 1 103,537 isoform 2 37,499 Cytochrome P450, Family 2, Subfamily J, polypeptide 2 (CYP2J2/1573) 57,611 Carboxypeptidase E (CPE/1363) 53,151 Fibronectin 1 (FN1/2335) different isoforms 1. 262,607 2. 71,943 3. 259,198 4. 222,944 5. 243,316 6. 240,477 7. 268,894 8. 252,793 9. 246,670 10. 239,608 11. 262,388 12. 221,274 13. 249,304 14. 249,384 Fassbender et al . Reproductive Biology and Endocrinology 2011, 9:44 http://www.rbej.com/content/9/1/44 © 2011 Fassbender et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. peaks observed after proteomic analysis, which are expensive and labour intense requiring High-performance liquid chromatography or high-pressure liquid chromato- graphy (HPLC) and matrix assisted laser desorption ioni- zation Time-of-Flight-Mass Spectrometry (MALDI-TOF/ TOF MS).” Author details 1Leuven University Fertility Centre, Department of Obstetrics & Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium. 2Division of Reproductive Health and Biology, Institute of Primate Research, P.O. Box 24481-00502 Karen, Nairobi, Kenya. 3Biochemistry Section, Department of Molecular Cell Biology, Campus Gasthuisberg, Leuven, Belgium. 4Department of Electrical Engineering, ESAT-SCD, K.U.Leuven, Kasteelpark-Arenberg 10, B-3001 Heverlee, Belgium. Received: 29 March 2011 Accepted: 6 April 2011 Published: 6 April 2011 References 1. Fassbender Amelie, Simsa Peter, Kyama MCleophas, Waelkens Etienne, Mihalyi Attila, Meuleman Christel, Gevaert Olivier, Van de Plas Raf, de Moor Bart, D ’Hooghe MThomas: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis. Reproductive Biology and Endocrinology 2010, 8:123. 2. Sherwin JR, Sharkey AM, Mihalyi A, Simsa P, Catalano RD, D ’Hooghe TM: Global gene analysis of late secretory phase, eutopic endometrium does not provide the basis for a minimally invasive test of endometriosis. Hum Reprod 2008, 23(5):1063-1068. doi:10.1186/1477-7827-9-44 Cite this article as: Fassbender et al .: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis. Reproductive Biology and Endocrinology 2011 9:44. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit Table 1 The representative molecular weights of the proteins identified in the mRNA Microarray study [Thirteen] [2] (Continued) 15. 272,302 Synuclein, gamma (SNCG/6623) 13,331 BAI1-associated protein 2 (BAIAP2/10458) different isoforms 1. 60,868 2. 59,014 3. 56,626 4. 57,359 5. 57,445 6. 57,430 Protocadherin 17 (PCDH17/27253) different isoforms 1. 126,229 2. 96,570 Protein tyrosine phosphatase, receptor type, R (PTPRR/5801) Alpha 73,834 Da Gamma 46,581Da Delta 51,046Da Fassbender et al . Reproductive Biology and Endocrinology 2011, 9:44 http://www.rbej.com/content/9/1/44 Page 2 of 2

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