Pengaruh Pemberian Ekstrak Meniran Terhadap Ekspresi MMP 9 Dan Luas Lesi Endometriosis Pada Mencit Model Endometriosis

Stefani Angel Kumalasari
In: Jurnal Biosains Pascasarjana · 2018 · vol. 20(1) , pp. 56 · doi:10.20473/jbp.v20i1.2018.56-65 · W2914884779
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This study investigated the effect of *Phyllanthus niruri* L. extract on MMP-9 expression and endometriosis lesion size in a mouse model, finding a significant reduction in both with extract treatment.

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The study investigated whether oral extracts of Phyllanthus niruri (meniran) at doses of 14, 28, and 56 mg/20 g body weight influence MMP-9 expression and the size of endometriosis lesions in a laboratory mouse model of endometriosis. Twenty-four female mice were used, with endometriosis induced for 14 days, followed by Na-CMC vehicle in controls and meniran extract in treatment groups; lesion area in the peritoneal cavity and MMP-9 expression were then assessed, with reported group means showing lower MMP-9 expression (K2–K4) and smaller lesion areas compared with the control (K1). The authors’ stated caveat is limited to the described experimental framework (an induced mouse model and specific dosing/regimen), which may constrain generalization beyond this setup. This paper is centrally about endometriosis — it evaluates meniran extract effects on MMP-9 expression and lesion area in an induced endometriosis mouse model.

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Abstract

AbstrakPada endometriosis, ekspresi MMP 9 ditemukan dalam konsentrasi yang lebih tinggi.Sekresi MMP 9 disebabkan oleh aktivasi ROS di rongga peritoneum dan menyebabkan peningkatan stres oksidatif. Peningkatan aktivitas MMP 9 pada endometriosis dapat memfasilitasi invasi yang mengakibatkan pembentukan lesi endometriosis. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak meniran (Phyllanthus niruri L.) pada dosis 14,28 dan 56 mg / 20 gBB terhadap ekspresi MMP 9 dan luas lesi endometriosis pada mencit model endometriosis. Penelitian ini merupakan penelitian eksperimental laboratorium. Dua puluh empat tikus betina digunakan sebagai sampel dan dibagi menjadi 4 kelompok: 1 kelompok kontrol dan 3 kelompok perlakuan. Keduanya, kelompok kontrol dan kelompok perlakuan diinduksi dan dijadikan model endometriosis selama 14 hari. 14 hari berikutnya, Na-CMC 0,5% diberikan kepada kelompok kontrol, sedangkan ekstrak Phyllanthus niruri L. pada dosis 14, 28 dan 56 mg / 20 gBB diberikan kepada kelompok perlakuan secara oral. Luas lesi endometriosis pada rongga peritoneum diperiksa. Hasil penelitian ini menunjukkan bahwa ada perbedaan yang signifikan pada ekspresi MMP 9 dengan rata-rata (± SD) K1, K2, K3, K4 sebesar 2.19±1.77, 0.19±0.13, 0.14±0.13, 0.08±0.14 dan luas lesi endometriosis pada K1, K2, K3, K4 adalah 120.04±100.09, 73.86±36.72, 69.08±15.73, 25.53±3.51. Kesimpulannya, ekspresi MMP 9 dan lesi endometriosis secara signifikan lebih rendah pada kelompok yang diobati dengan ekstrak Phyllanthus niruri L. herbal. Kata kunci : ekstrak meniran, endometriosis, ekspresi MMP 9, luas lesi endometriosis
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Pengaruh Pemberian Ekstrak Meniran Terhadap Ekspresi MMP 9 Dan Luas Lesi Endometriosis Pada Mencit Model Endometriosis Downloads Abstrak Pada endometriosis, ekspresi MMP 9 ditemukan dalam konsentrasi yang lebih tinggi.Sekresi MMP 9 disebabkan oleh aktivasi ROS di rongga peritoneum dan menyebabkan peningkatan stres oksidatif. Peningkatan aktivitas MMP 9 pada endometriosis dapat memfasilitasi invasi yang mengakibatkan pembentukan lesi endometriosis. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak meniran (Phyllanthus niruri L.) pada dosis 14,28 dan 56 mg / 20 gBB terhadap ekspresi MMP 9 dan luas lesi endometriosis pada mencit model endometriosis. Penelitian ini merupakan penelitian eksperimental laboratorium. Dua puluh empat tikus betina digunakan sebagai sampel dan dibagi menjadi 4 kelompok: 1 kelompok kontrol dan 3 kelompok perlakuan. Keduanya, kelompok kontrol dan kelompok perlakuan diinduksi dan dijadikan model endometriosis selama 14 hari. 14 hari berikutnya, Na-CMC 0,5% diberikan kepada kelompok kontrol, sedangkan ekstrak Phyllanthus niruri L. pada dosis 14, 28 dan 56 mg / 20 gBB diberikan kepada kelompok perlakuan secara oral. Luas lesi endometriosis pada rongga peritoneum diperiksa. Hasil penelitian ini menunjukkan bahwa ada perbedaan yang signifikan pada ekspresi MMP 9 dengan rata-rata (± SD) K1, K2, K3, K4 sebesar 2.19±1.77, 0.19±0.13, 0.14±0.13, 0.08±0.14 dan luas lesi endometriosis pada K1, K2, K3, K4 adalah 120.04±100.09, 73.86±36.72, 69.08±15.73, 25.53±3.51. Kesimpulannya, ekspresi MMP 9 dan lesi endometriosis secara signifikan lebih rendah pada kelompok yang diobati dengan ekstrak Phyllanthus niruri L. herbal. Kata kunci : ekstrak meniran, endometriosis, ekspresi MMP 9, luas lesi endometriosis DAFTAR PUSTAKA Arumugam K and Dip YC. 1995. 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