The effect of hyperbaric oxygen therapy in the inflammatory response in a mouse model of endometriosis: An experimental study

Dedy Syahrizal, Mustika C, Cut Mustika, Cut Rizki Mustika, Ayu Puspita N, Nanda Ayu Puspita, Mohammad Guritno Suryokusumo, Guritno Suryokusumo M, Hendy Hendarto, Hendarto H
International journal of reproductive biomedicine · 2022 · vol. 20(5) , pp. 347–356 · doi:10.18502/ijrm.v20i5.11049 · PMID:35911854 · PMC9334893 · W4282976195
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⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

Hyperbaric oxygen therapy reduced peritoneal inflammation in endometriosis-induced mice by decreasing NFκB expression.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-21 · read from full text ⓘ

This experimental study used 24 female Swiss albino mice with endometriosis induced by intraperitoneal xenotransplantation of endometrial cells, then compared groups with no treatment, HBOT given for 10 days, or HBOT omitted, assessing peritoneal inflammation histopathologically and nuclear factor kappa B (NF-κB) expression by immunohistochemistry. HBOT (100% O2, 2.4 atm, 3×30 min with a 5-min air break for 10 consecutive days) significantly lowered peritoneal inflammation scores in the HBOT group versus both pre-test and non-HBOT post-test groups, and NF-κB expression was highest in controls and reduced after HBOT; NF-κB expression also correlated positively with inflammation severity (Spearman r = 0.670). A key limitation is that the analysis used semiquantitative scoring and a small sample size (n=8 per group), which constrains precision of the measured inflammatory and NF-κB changes. This paper is centrally about endometriosis — it tests how hyperbaric oxygen therapy alters peritoneal inflammation and NF-κB activity in a mouse model of endometriosis.

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Abstract

BACKGROUND: Endometriosis pathogenesis is related to the inflammation shown by the secretion of pro-inflammatory mediators. This hypoxia condition can stimulate this condition. OBJECTIVE: To investigate the effect of hyperbaric oxygen therapy (HBOT) on the inflammation reaction of endometriosis-induced mice. MATERIALS AND METHODS: The animals were designated into 3 groups: I) the pre-test group, II) the post-test group receiving the HBOT, and III) the post-test group without HBOT. All groups were subjected to induction of endometriosis by xenotransplantation for 15 days. HBOT was given 30 min 3 times a day for 10 days. The evaluation of the HBOT effect was conducted by examining the endometrial tissue. The inflammation level was evaluated using the Klopfleisch semiquantitative scoring system (index remmele scale), whilst the expression of nuclear factor kappa (NF κ B) beta was measured by immunohistochemical staining. RESULTS: The results showed that group I demonstrated the highest level of inflammation degree (9.41 ± 1.99) compared to the post-test groups (group II: 1.60 ± 0.53; group III: 2.42 ± 0.53). The HBOT-groups was found to have the lowest inflammation level compared to the non-HBOT group (p = 0.020). The results demonstrated that HBOT lowered the peritoneal inflammation degree caused by the endometrial lesion in mice. NF κ B expression on the post-test groups was significantly decreased, compared to the pre-test group (p ≤ 0.001), with a strong correlation between the NF κ B expression and the peritoneal inflammation level (p ≤ 0.001, r = 0.670). CONCLUSION: HBOT significantly reduced the inflammation level on the endometrial lesion in mice, involving the NF κ B pathway.
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Section

Our study showed that HBO therapy reduced the inflammatory state in endometrial lesions, possibly via the alteration of the NF κ B pathway. Further investigation in the duration and dose of HBOT is needed to elucidate the molecular mechanism of HBOT in endometriosis. Our results have shown that HBOT significantly reduced the inflammation level on the endometrial lesions, with a low level of NF κ B expression.

Coi Statement

The authors declare that they have no competing interest.

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Condition tags

endometriosis

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