Effect of human seminal fluid on the growth of endometrial cells of women with endometriosis

Khaleque Newaz Khan, Khaleque N Khan, Khan KN, Kitajima M, Michio Kitajima, Hiraki K, Koichi Hiraki, Akira Fujishita, Fujishita A, Ichiro Sekine, Sekine I, Ishimaru T, Tadayuki Ishimaru, Masuzaki H, Hideaki Masuzaki
European journal of obstetrics, gynecology, and reproductive biology · 2010 · vol. 149(2) , pp. 204–209 · doi:10.1016/j.ejogrb.2009.12.022 · PMID:20092935 · W1993043619
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Human seminal plasma significantly stimulated the growth of endometrial cells from women with endometriosis, with higher proliferation observed compared to women without the condition.

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The study investigated how human seminal fluid affects the growth of endometrial cells derived from women with endometriosis versus women without endometriosis, using serum, peritoneal fluid, and eutopic/ectopic endometrial tissue specimens collected during laparoscopy, alongside seminal plasma collected from 18 healthy men. Seminal plasma significantly stimulated proliferation of epithelial and stromal cells from eutopic and ectopic endometria of women with endometriosis (2–3-fold), and proliferation in eutopic-derived gland and stromal cells was higher in women with endometriosis than in those without. Although PGE2, HGF, and estradiol levels in seminal plasma were variable, PGE2 and HGF were significantly higher in seminal plasma than in peritoneal fluid or serum; blocking single pathways with anti-PGE2 or anti-HGF alone did not suppress growth, whereas combined blockade of anti-PGE2 plus anti-HGF (and in some conditions plus tamoxifen/raloxifene) did. This paper is centrally about endometriosis — it tests how human seminal fluid components promote proliferation of eutopic and ectopic endometrial cells specifically in women with endometriosis.

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Abstract

ObjectivesWe investigated the effect of human seminal fluid on the growth of endometrial cells derived from women with and without endometriosis.Study designSeminal plasma (SP) was collected from 18 healthy fertile men. Serum, peritoneal fluid (PF) and tissue specimens of eutopic and ectopic endometrium were collected from 45 women with endometriosis and 20 women without endometriosis during laparoscopic surgery. Prostaglandin (PG) E2, hepatocyte growth factor (HGF), and estradiol (E2) levels in each sample of SP, serum and PF were measured by enzyme-linked immunosorbent assay. The growth pattern of cells derived from eutopic and ectopic endometria in response to SP was examined by 5-bromo-2-deoxyuridine (BrdU) incorporation assay.ResultsSeminal plasma was able to significantly stimulate the growth of epithelial cells and stromal cells derived from the eutopic and ectopic endometria of women with endometriosis (2-3-fold) when compared with control media. The SP-promoted proliferation of both gland cells and stromal cells derived from eutopic endometria was also remarkably higher in women with endometriosis than that of women without endometriosis. Although levels of PGE2, HGF and E2 in SP were variable when compared with other body fluids, the levels of PGE2 and HGF in SP were significantly higher than those in either peritoneal fluid or serum of women with or without endometriosis. Pretreatment of cells with individual anti-PGE2 antibody, anti-HGF antibody and two selective estrogen receptor modulators, tamoxifen and raloxifene was unable to suppress SP-mediated growth of endometrial cells. However, pretreatment of cells with combined anti-PGE2 antibody plus anti-HGF antibody or combined anti-PGE2 antibody plus anti-HGF antibody plus tamoxifen or raloxifene was able to significantly suppress SP-promoted growth of eutopic and ectopic endometrial cells.ConclusionHuman seminal fluid enriched with different macromolecules may promote the growth of endometrial cells derived from women with endometriosis. Our findings may suggest some detrimental effect of unprotected sexual intercourse in women with endometriosis.
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WEKO3 アイテム Effect of human seminal fluid on the growth of endometrial cells of women with endometriosis. http://hdl.handle.net/10069/22904 http://hdl.handle.net/10069/22904e76cc205-7f77-435f-a245-8487ed043cfc | 名前 / ファイル | ライセンス | アクション | |---|---|---| | EJOGRB149_204.pdf (321.0 kB) | | | アイテムタイプ | 学術雑誌論文 / Journal Article(1) | ||||||||||||||||||| |---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---| | 公開日 | 2010-02-10 | ||||||||||||||||||| | タイトル | |||||||||||||||||||| | タイトル | Effect of human seminal fluid on the growth of endometrial cells of women with endometriosis. | ||||||||||||||||||| | 言語 | |||||||||||||||||||| | 言語 | eng | ||||||||||||||||||| | キーワード | |||||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||||| | 主題 | Cell growth | ||||||||||||||||||| | キーワード | |||||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||||| | 主題 | Endometriosis | ||||||||||||||||||| | キーワード | |||||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||||| | 主題 | Macromolecules | ||||||||||||||||||| | キーワード | |||||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||||| | 主題 | Peritoneal fluid | ||||||||||||||||||| | キーワード | |||||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||||| | 主題 | Seminal plasma | ||||||||||||||||||| | 資源タイプ | |||||||||||||||||||| | 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | ||||||||||||||||||| | 資源タイプ | journal article | ||||||||||||||||||| | 著者 | Khan, Khaleque Newaz × Khan, Khaleque Newaz × Kitajima, Michio × Hiraki, Koichi × Fujishita, Akira × Sekine, Ichiro × Ishimaru, Tadayuki × Masuzaki, Hideaki | ||||||||||||||||||| | 抄録 | |||||||||||||||||||| | 内容記述タイプ | Abstract | ||||||||||||||||||| | 内容記述 | OBJECTIVES: We investigated the effect of human seminal fluid on the growth of endometrial cells derived from women with and without endometriosis. STUDY DESIGN: Seminal plasma (SP) was collected from 18 healthy fertile men. Serum, peritoneal fluid (PF) and tissue specimens of eutopic and ectopic endometrium were collected from 45 women with endometriosis and 20 women without endometriosis during laparoscopic surgery. Prostaglandin (PG) E2, hepatocyte growth factor (HGF), and estradiol (E2) levels in each sample of SP, serum and PF were measured by enzyme-linked immunosorbent assay. The growth pattern of cells derived from eutopic and ectopic endometria in response to SP was examined by 5-bromo-2-deoxyuridine (BrdU) incorporation assay. RESULTS: Seminal plasma was able to significantly stimulate the growth of epithelial cells and stromal cells derived from the eutopic and ectopic endometria of women with endometriosis (2-3-fold) when compared with control media. The SP-promoted proliferation of both gland cells and stromal cells derived from eutopic endometria was also remarkably higher in women with endometriosis than that of women without endometriosis. Although levels of PGE2, HGF and E2 in SP were variable when compared with other body fluids, the levels of PGE2 and HGF in SP were significantly higher than those in either peritoneal fluid or serum of women with or without endometriosis. Pretreatment of cells with individual anti-PGE2 antibody, anti-HGF antibody and two selective estrogen receptor modulators, tamoxifen and raloxifene was unable to suppress SP-mediated growth of endometrial cells. However, pretreatment of cells with combined anti-PGE2 antibody plus anti-HGF antibody or combined anti-PGE2 antibody plus anti-HGF antibody plus tamoxifen or raloxifene was able to significantly suppress SP-promoted growth of eutopic and ectopic endometrial cells. CONCLUSION: Human seminal fluid enriched with different macromolecules may promote the growth of endometrial cells derived from women with endometriosis. Our findings may suggest some detrimental effect of unprotected sexual intercourse in women with endometriosis. | ||||||||||||||||||| | 書誌情報 | European journal of obstetrics, gynecology, and reproductive biology 巻 149, 号 2, p. 204-209, 発行日 2010-04 | ||||||||||||||||||| | 出版者 | |||||||||||||||||||| | 出版者 | Elsevier Ireland Ltd | ||||||||||||||||||| | ISSN | |||||||||||||||||||| | 収録物識別子タイプ | ISSN | ||||||||||||||||||| | 収録物識別子 | 03012115 | ||||||||||||||||||| | EISSN | |||||||||||||||||||| | 収録物識別子タイプ | ISSN | ||||||||||||||||||| | 収録物識別子 | 1872-7654 | ||||||||||||||||||| | 書誌レコードID | |||||||||||||||||||| | 収録物識別子タイプ | NCID | ||||||||||||||||||| | 収録物識別子 | AA00639654 | ||||||||||||||||||| | PubMed番号 | |||||||||||||||||||| | 関連タイプ | isVersionOf | ||||||||||||||||||| | 識別子タイプ | PMID | ||||||||||||||||||| | 関連識別子 | 20092935 | ||||||||||||||||||| | DOI | |||||||||||||||||||| | 関連タイプ | isVersionOf | ||||||||||||||||||| | 識別子タイプ | DOI | ||||||||||||||||||| | 関連識別子 | 10.1016/j.ejogrb.2009.12.022 | ||||||||||||||||||| | 権利 | |||||||||||||||||||| | 権利情報 | Copyright c 2010 Elsevier Ireland Ltd All rights reserved. | ||||||||||||||||||| | 著者版フラグ | |||||||||||||||||||| | 出版タイプ | AM | ||||||||||||||||||| | 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | ||||||||||||||||||| | 引用 | |||||||||||||||||||| | 内容記述タイプ | Other | ||||||||||||||||||| | 内容記述 | European journal of obstetrics, gynecology, and reproductive biology, 149(2), pp.204-209; 2010 |

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Condition tags

endometriosis

MeSH descriptors

Cell Proliferation Endometriosis Endometrium Semen Adult Cell Proliferation Dinoprostone Dinoprostone Endometriosis Endometrium Endometrium Estradiol Estradiol Female Hepatocyte Growth Factor Hepatocyte Growth Factor Humans Male Selective Estrogen Receptor Modulators Selective Estrogen Receptor Modulators

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