Analysis of DNA methylation and its correlation with mRNA expression of epidermal growth factor receptor encoding for cytoskeleton regulating protein in peritoneal endometriosis tissue

Annisah Zahrah, Raden Muharam, M. Luky Satria Marwali, A Sururi, Achmad Kemal Harzif, Gita Pratama, Ocktariyana Ocktariyana, Asmarinah Asmarinah
In: IOP Conference Series: Earth and Environmental Science · 2020 · vol. 457(1) , pp. 012076 · doi:10.1088/1755-1315/457/1/012076 · W3013714150
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This study analyzed EGFR gene DNA methylation and mRNA expression in endometriosis tissue, finding increased EGFR mRNA expression unrelated to DNA methylation alterations.

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This study investigated the relationship between DNA methylation and mRNA expression of the epidermal growth factor receptor (EGFR) gene in peritoneal endometriosis tissue. Researchers analyzed samples from 20 patients with peritoneal endometriosis and 20 non-endometriosis controls using Methylation Specific PCR and qRT-PCR to assess genetic modifications and transcript levels. The results showed significantly increased DNA methylation and elevated EGFR mRNA expression in endometriosis tissues compared to normal endometrium, yet no significant correlation existed between these two variables. The authors concluded that the upregulation of EGFR mRNA in endometriosis is not driven by changes in DNA methylation, suggesting other mechanisms contribute to its pathogenesis. This paper is centrally about endometriosis — specifically examining molecular pathways involved in peritoneal lesions.

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Abstract

Abstract It has been known that the EGFR have the role for regulation the cytoskeleton activity and its expression increased in endometriosis tissue. The aim of this study was to evaluate the DNA methylation of the EGFR gene that might cause the alteration of its mRNA expression in peritoneal endometriosis tissue. Samples were peritoneal endometriosis tissue from 20 endometriosis patients and 20 female of non-endometriosis patients. The DNA methylation of the EGFR gene was analyzed by the method of Methylation Specific PCR and ImageJ software, while its expression of mRNA were analyzed by the method of qRT-PCR. The DNA methylation in the EGFR gene in peritoneal endometriosis tissues increased compared to normal endometrial tissues (peritoneal endometriosis tissue = 56%, normal endometriosis tissue = 19%). The expression of mRNA EGFR gene in endometriosis peritoneal tissues was 1.341 fold increased relative to normal endometrium. There is no significant correlation between the DNA methylation with expression of mRNA EGFR (p = 0.947 and r = -0.016). Increasing of EGFR mRNA expression in endometriosis tissue that was not caused by alteration of its DNA methylation, have to play a role in the pathogenesis of endometriosis.
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Abstract It has been known that the EGFR have the role for regulation the cytoskeleton activity and its expression increased in endometriosis tissue. The aim of this study was to evaluate the DNA methylation of the EGFR gene that might cause the alteration of its mRNA expression in peritoneal endometriosis tissue. Samples were peritoneal endometriosis tissue from 20 endometriosis patients and 20 female of non-endometriosis patients. The DNA methylation of the EGFR gene was analyzed by the method of Methylation Specific PCR and ImageJ software, while its expression of mRNA were analyzed by the method of qRT-PCR. The DNA methylation in the EGFR gene in peritoneal endometriosis tissues increased compared to normal endometrial tissues (peritoneal endometriosis tissue = 56%, normal endometriosis tissue = 19%). The expression of mRNA EGFR gene in endometriosis peritoneal tissues was 1.341 fold increased relative to normal endometrium. There is no significant correlation between the DNA methylation with expression of mRNA EGFR (p = 0.947 and r = -0.016). Increasing of EGFR mRNA expression in endometriosis tissue that was not caused by alteration of its DNA methylation, have to play a role in the pathogenesis of endometriosis. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

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endometriosis

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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