The Content of Cytokines IL-6, IL-8, TNF-α, IL-4 and the Level of CD86 and CD163 Expression in Peritoneal Fluid Macrophages Has a Reverse Correlation with the Degree of Severity of External Genital Endometriosis

Abstract— Concentrations of eight different cytokines and the level of CD86 and CD163 expression by macrophages were studied in the peritoneal fluid of women with endometriosis. The concentration of both pro-inflammatory (IL-6, IL-8, TNF-α) and anti-inflammatory cytokines (IL-4) as well as the level of macrophage expression of the proinflammatory marker CD86 and anti-inflammatory marker CD163 increased in women with mild external genital endometriosis (stage 1−2), and did not differ from the control group in women with severe endometriosis (3−4 stage). The content of IL-2, IL-10, CM-CSF and IFN-γ in the peritoneal fluid of women with endometriosis insignificantly differed from the control group. The results of the study indicate that the development of external genital endometriosis may be associated with insufficient pro-inflammatory and also anti-inflammatory activity of peritoneal fluid macrophages. Similar content being viewed by others REFERENCES Giudice, L.C. and Kao, L.C., Lancet, 2004, vol. 364, no. 9447, pp. 1789−1799. Brosens, I. and Benagiano, G., Indian J. Med. Res., 2011, vol. 133, no. 6, pp. 581−593. Acién, P. and Velasco, I., ISRN Obstetrics Gynecology, 2013, vol. 2013, no. 4, 242149. Krasnyi, A.M., Volgina, N.E., Sadekova, A.A., Shchipitsyna, V.S., Sukhih, G.T., Adamyan, L.V., and Ozernyuk, N.D., Izvestiya Rossiyskoy Akademii Nauk. Ser. Biol., 2017, no. 2, pp. 77−80. https://doi.org/10.7868/S0002332917020072 van Furth, R., Raeburn, J.A., and van Zwet, T.L., Blood, 1979, vol. 54, no. 2, pp. 485−500. Gazvani, R., and Templeton, A., Reproduction, 2002, vol. 123, pp. 217−226. Riccio, L.D.G.C., Santulli, P., Marcellin, L., Abrão, M.S., Batteux, F., and Chapron, C., Best Practice & Research Clinical Obstetrics & Gynaecology, 2018, vol. 50, pp. 39−49. Sica, A. and Mantovani, A., J. Clin. Invest., 2012, vol. 122, no. 3, pp. 787−795. Yao, Y., Xu, X.H., and Jin, L., Frontiers Immunol., 2019, vol. 10, 792. Bacci, M., Capobianco, A., Monno, A., Cottone, L., Di Puppo, F., Camisa, B., Mariani, M., Brignole, C., Ponzoni, M., Ferrari, S., Panina-Bordignon, P., Manfredi, A.A., and Rovere-Querini, P., Am. J. Pathol., 2009, vol. 175, no. 2, pp. 547−556. Itoh, F., Komohara, Y., Takaishi, K., Honda, R., Tashiro, H., Kyo, S., Katabuchi, H., and Takeya, M., Fertility & Sterility, 2013, vol. 99, no. 6, pp. 1705−1713. Cameron, M.J. and Kelvin, D.J., Adv. Exper. Med. Bio-l., 2003, vol. 520, pp. 8−32. Cheong, Y.C., Shelton, J.B., Laird, S.M., Rich-mond, M., Kudesia, G., Li, T.C., and Ledger, W.L., Hum. Reprod., 2002, vol. 17, no. 1, pp. 69−75. Pizzo, A., Salmeri, F.M., Ardita, F.V., Sofo, V., Tripepi, M., and Marsico, S., Gynecologic Obstetric Invest., 2002, vol. 54, no. 2, pp. 82−87. Harada, T., Yoshioka, H., Yoshida, S., Iwabe, T., Onohara, Y., Tanikawa, M., and Terakawa, N., Am. J. Obstetr. Gynecol., 1997, vol. 176, no. 3, pp. 593−597. Volgina, N.E., Shchipitsyna, V.S., Khilkevich, E.G., Chuprynin, V.D., Adamyan, L.V., Ozernyuk, N.D., and Krasnyi, A.M., Immunologiya, 2016, vol. 37, no. 3, pp. 181−184. https://doi.org/10.18821/0206-4952-2016-37-3-181-184 Ho, H.N., Wu, M.Y., Chao, K.H., Chen, C.D., Chen, S.U., and Yang, Y.S., Hum. Reprod., 1997, vol. 12, no. 11, pp. 2528−2533. Gazvani, M.R., Christmas, S., Quenby, S., Kirwan, J., Johnson, P.M., and Kingsland, C.R., Hum. Reprod., 1998, vol. 13, no. 7, pp. 1957−1961. Saiddanesh, Sh.F., Chuprynin, V.D., Khilkevich, E.G., Buralkina, N.A., Pavlovich, S.V., Danilov, A.Yu., and Chursin, V.V., Akusherstvo i ginekologiya, 2017, no. 5, pp. 39−43. https://doi.org/10.18565/aig.2017.5.39-43 Liao, C.T., Andrews, R., Wallace, L.E., Khan, M.W., Kift-Morgan, A., Topley, N., Fraser, D.J., and Taylor, P.R., Kidney International, 2017, vol. 91, no. 5, pp. 1088−1103. Funding The study was performed within the framework of the State Assignment “Development Approaches for Non-Invasive Diagnosis of Endometriosis Based on Omics Technologies,” the registration number AAAA-A19-119 021 490 132-9. Author information Authors and Affiliations Corresponding author Ethics declarations The study was approved by the Ethics Committee of the Academician Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology. Before collecting the biological material, all women signed an informed consent to participate in this clinical study. The authors declare that they have no conflict of interest. Additional information Translated by A. Medvedev Rights and permissions About this article Cite this article Krasnyi, A.M., Sadekova, A.A., Sefihanov, T.G. et al. The Content of Cytokines IL-6, IL-8, TNF-α, IL-4 and the Level of CD86 and CD163 Expression in Peritoneal Fluid Macrophages Has a Reverse Correlation with the Degree of Severity of External Genital Endometriosis. Biochem. Moscow Suppl. Ser. B 14, 52–56 (2020). https://doi.org/10.1134/S1990750820010096 Received: Revised: Accepted: Published: Version of record: Issue date: DOI: https://doi.org/10.1134/S1990750820010096