Immunohistochemical localization of selected pro-inflammatory factors in uterine myomas and myometrium in women of various ages

Andrzej Plewka, Paweł Madej, Danuta Plewka, Anna Kowalczyk, Adam Miśkiewicz, Piotr Wittek, Tomasz Leks, Rafał Bilski
In: Folia Histochemica et Cytobiologica · 2013 · vol. 51(1) , pp. 73–83 · doi:10.5603/fhc.2013.0011 · PMID:23690221 · W2155511584
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This study examined the expression of pro-inflammatory factors in uterine myomas and myometrium across different age groups, finding higher expression in myomas that varied with myoma size and patient age, but without increased nuclear NF-kappaB activation.

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The study investigated immunohistochemical expression of pro-inflammatory mediators—NF-κB, IL-1β, TNF-α, COX-2, and iNOS—in uterine myomas and adjacent myometrium from women across reproductive and perimenopausal ages. Using semiquantitative immunohistochemistry, the authors found higher expression of the examined proteins in myomas than in control myometrium, with levels depending on myoma size and women’s age. A key caveat was that cytoplasmic NF-κB in myomas showed no association with myoma size, and there were no significant differences in the number of stained nuclei between myoma and control groups, indicating no nuclear activation of NF-κB p65 accompanying the pro-inflammatory expression. This paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Uterine myomas represent one of the most frequently manifested benign tumors in women. They originate from smooth muscle cells of myometrium or its blood vessels. Many studies suggest that inflammation and pro-inflammatory factors may play a role in the carcinogenesis with an involvement of the transcription factor NF-kappaB which activity can be controlled by various environmental factors, including many cytokines. The aim of the study was to investigate the expression of NF-B, interleukin-1β (IL-1β), tumor necrosis factor a (TNF-α), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) in myometrium and uterine myomas of women of various age. The expression of NF-kappaB, selected cytokines and enzymes was estimated in women of reproductive or perimenopausal age by semiquantitative immunohistochemistry. The expression of the examined proteins was higher in myomas than in control myometrium and was dependent on the size of myomas and the age of women. However, the expression of the cytoplasmic NF-kappaB observed in uterine myomas was independent on the size of myomas and no significant differences were observed in the number of stained nuclei between control and myoma groups. Thus, the expression of proinflammatory factors in myomas was not accompanied by the nuclear activation of NF-kappaB p65. The results of our study indicate that the examined factors may be involved in the pathogenesis of benign tumors and not only malignant diseases.
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Abstract

Uterine myomas represent one of the most frequently manifested benign tumors in women. They originate from smooth muscle cells of myometrium or its blood vessels. Many studies suggest that inflammation and pro-inflammatory factors may play a role in the carcinogenesis with an involvement of the transcription factor NF-kappaB which activity can be controlled by various environmental factors, including many cytokines. The aim of the study was to investigate the expression of NF-B, interleukin-1β (IL-1β), tumor necrosis factor a (TNF-α), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) in myometrium and uterine myomas of women of various age. The expression of NF-kappaB, selected cytokines and enzymes was estimated in women of reproductive or perimenopausal age by semiquantitative immunohistochemistry. The expression of the examined proteins was higher in myomas than in control myometrium and was dependent on the size of myomas and the age of women. However, the expression of the cytoplasmic NF-kappaB observed in uterine myomas was independent on the size of myomas and no significant differences were observed in the number of stained nuclei between control and myoma groups. Thus, the expression of proinflammatory factors in myomas was not accompanied by the nuclear activation of NF-kappaB p65. The results of our study indicate that the examined factors may be involved in the pathogenesis of benign tumors and not only malignant diseases. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 73–83)

Keywords

womenuterine myomasmyometriumNF-kappaBIL-1βTNF-αCOX-2iNOSimmunohistochemistryage

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