miR-424-5p Combined with miR-17-5p Has High Diagnostic Efficacy for Endometriosis

Chunli Lin, Saili Zeng, Miaojie Li
In: Research Square · 2021 · doi:10.21203/rs.3.rs-1116541/v1 · W4200107259
preprint OA: green CC0
📄 Open PDF View on OpenAlex View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

Serum miR-424-5p combined with miR-17-5p demonstrated high diagnostic efficacy for endometriosis, with a combined AUC of 0.938, outperforming individual miRNA detection.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This study evaluated whether serum levels of microRNA (miR) miR-17-5p and miR-424-5p, alone and combined, have diagnostic value for endometriosis by comparing 80 reproductive-age women with pathology-confirmed endometriosis to 80 healthy reproductive-age controls. Using RT-qPCR on whole-blood-derived serum, the authors found both miRNAs were significantly downregulated in endometriosis, and ROC analysis showed higher diagnostic efficacy for their combination (AUC 0.938; 93.8% sensitivity and 88.7% specificity) than for either miRNA alone. They also measured VEGFA and inflammatory cytokines (IL-4, IL-6), reporting that these were increased in endometriosis and inversely associated with miR-17-5p and miR-424-5p, with negative correlations between the miRNAs and clinical features such as dysmenorrhea, infertility, pelvic pain, and rASRM stage. The paper’s key limitation is that it is a preprint and the included biomarker analysis appears limited to a single case-control cohort. This paper is centrally about endometriosis—specifically, combined diagnostic performance of serum miR-17-5p and miR-424-5p for endometriosis.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Abstract Purpose: Endometriosis (EMT) is a chronic benign disease with high prevalence. This study investigated the diagnostic value of serum miR-17-5p, miR-424-5p, and their combined expressions for EMT. Methods: A total of 80 EMT patients of reproductive age were included as the study subjects, and another 80 healthy women of reproductive age were selected as the control group. The whole blood samples of enrolled subjects were collected and clinical characteristics were recorded. The miR-17-5p, miR-424-5p, VEGFA, IL-4, and IL-6 levels in the serum were measured. ROC curve was used to evaluate the diagnostic efficacy of miR-17-5p and miR-424-5p expressions for EMT. Pearson correlation was performed to analyze the correlation of miR-17-5p and miR-424-5p with clinical indexes in EMT patients. Results: miR-17-5p and miR-424-5p were significantly downregulated in EMT patients. For the diagnosis of EMT, the AUC of miR-17-5p was 0.865 and cutoff value was 0.890 (91.3% sensitivity and 85% specificity), the AUC of miR-424-5p was 0.737 and cutoff value was 0.915 (98.8% sensitivity and 61.2% specificity), the AUC of miR-424-5p combined with miR-17-5p was 0.938 and cutoff value was 2.205 (93.8% sensitivity and 88.7% specificity), with the diagnostic efficacy higher than miR-424-5p or miR-17-5p alone. The expressions of miR-17-5p and miR-424-5p were negatively correlated with dysmenorrhea, infertility, pelvic pain, and rASRM stage, but not with age, BMI, menstrual disorder, and nulliparity. VEGFA, IL-4, and IL-6 were remarkably increased in EMT patients, and both were inversely associated with miR-17-5p and miR-424-5p. Conclusion: miR-424-5p combined with miR-17-5p has high diagnostic efficacy for EMT.

My notes (saved in your browser only)

Outcome instruments

rASRM

Condition tags

endometriosisdysmenorrheainfertility

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (28)

Source provenance

europepmc
last seen: 2026-06-04T01:45:00.660873+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK