Effect of 5-aminolevulinic acid (ALA) doses and oestrogen/progesterone on protoporphyrin IX (ppIX) accumulation in human endometrial epithelial cells.

W Butowska; Butowska W; W Warchoł; Warchoł W; Wojciech Warchoł; Nowak-Markwitz E; Ewa Nowak‐Markwitz; E Nowak-Markwitz; Wołuń-Cholewa M; Maria Wołuń-Cholewa; M Wołuń-Cholewa

Effect of 5-aminolevulinic acid (ALA) doses and oestrogen/progesterone on protoporphyrin IX (ppIX) accumulation in human endometrial epithelial cells.

W Butowska, Butowska W, W Warchoł, Warchoł W, Wojciech Warchoł, Nowak-Markwitz E, Ewa Nowak‐Markwitz, E Nowak-Markwitz, Wołuń-Cholewa M, Maria Wołuń-Cholewa, M Wołuń-Cholewa

Roczniki Akademii Medycznej w Bialymstoku (1995) · 2004 · vol. 49 Suppl 1 , pp. 123–5 · PMID:15638395 · W2136918309

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Estradiol and progesterone increased 5-aminolevulinic acid-induced protoporphyrin IX accumulation in human endometrial epithelial cells in a time-dependent manner.

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Abstract

Endometriosis represents one of the most frequent causes of restricted fecundity. Despite the progress in medicine, appropriate diagnosis and treatment pose significant problems. The aim of this study was to evaluate ALA-induced PpIX fluorescence of normal endometrial epithelial cells for the diagnosis of endometriosis. PpIX-fluorescence was measured after stimulation with estradiol-17 beta (E2) or with estradiol-17 beta (E2) and progesterone (P) and after incubation with ALA under a confocal microscope. The epithelial cells showed a significantly higher fluorescence of PpIX in the course of 24 and 48h incubation with hormones, than the cells without stimulation. After 72h, a significant decrease in cellular PpIX concentration was noted. The results suggested that E2 and P were required to convert ALA to PpIX in epithelial cells and increased PpIX concentration in a time-dependent fashion.

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Condition tags

endometriosis

MeSH descriptors

Aminolevulinic Acid Endometrium Epithelial Cells Estradiol Progesterone Protoporphyrins Aminolevulinic Acid Endometrium Endometrium Endometrium Epithelial Cells Epithelial Cells Estradiol Female Humans Progesterone Protoporphyrins

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Cites (3)

References (9)

Endometriosis: Correlation between histologic and visual findings at laparoscopy via openalex
Etiology and epidemiology of endometriosis via openalex
Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro. via openalex
W2067949612 via openalex
W2091067431 via openalex
W1487391398 via openalex
W2115499196 via openalex
W1996356730 via openalex
W2063990873 via openalex

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[1] Endometriosis: Correlation between histologic and visual findings at laparoscopy 2001

[2] Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro. 1994

[3] Etiology and epidemiology of endometriosis 1990