Serum metabolic profiling study of endometriosis by using wooden-tip electrospray ionization mass spectrometry

Bicheng Yang, Faying Liu, Liqun Wang, Yang Zou, Feng Wang, Wei Deng, Xi‐di Wan, Xiao Yang, Ming He, Ouping Huang
In: Analytical Methods · 2015 · vol. 7(15) , pp. 6125–6132 · doi:10.1039/c5ay01312g · W1898759981
article OA: closed CC0 ⤵ 6 in-corpus citations
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

This study established a rapid serum metabolic profiling method using WT-ESI-MS for endometriosis analysis without extensive sample preparation or chromatography.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 · read from full text ⓘ

The study developed a high-throughput serum metabolite fingerprinting method using wooden-tip electrospray ionization mass spectrometry (WT-ESI-MS) with little sample pre-treatment, no chromatography, and instrument cycle times under 5 minutes. Serum from endometriosis patients and healthy controls was analyzed with direct WT-ESI-MS using high-resolution ESI-Q-TOF-MS, and multivariate data analysis plus MS/MS were used to identify metabolites that differed between groups. Global metabolic profiling clearly distinguished endometriosis patients from healthy controls, and ten metabolites (up- or down-regulated) were reported as potential biomarkers linked to pathways including steroid hormone biosynthesis, glycerophospholipid and sphingolipid metabolism, pyruvate metabolism, bile acid biosynthesis, and androgen/estrogen metabolism. The paper’s main caveat is that it presents identified metabolites and pathway associations without providing detailed validation limitations within the abstract. This paper is centrally about endometriosis — it uses WT-ESI-MS serum metabolic profiling to distinguish patients from healthy controls and identify potential metabolite biomarkers and related metabolic pathways.

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Abstract

A high throughput metabolite fingerprinting tool based on WT-ESI-MS has been established for the serum metabolic profiling study of endometriosis with little sample pre-treatment, no chromatography and instrument cycle times of less than 5 min.
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Serum metabolic profiling study of endometriosis by using wooden-tip electrospray ionization mass spectrometry Abstract A high throughput metabolite fingerprinting tool based on wooden-tip electrospray ionization mass spectrometry (WT-ESI-MS) has been established for the serum metabolic profiling study of endometriosis with little sample pre-treatment, no chromatography and instrument cycle times of less than 5 min. Serum samples from endometriosis patients and healthy controls were analyzed by direct WT-ESI-MS with a high resolution ESI-Q-TOF-MS. The resulting data were analyzed by multivariate data analysis. MS/MS experiments were carried out to identify potential biomarkers. Global metabolic profiling and subsequent multivariate analysis clearly distinguished endometriosis patients from healthy controls. A total of ten metabolites, up-regulated or down-regulated, were identified which contribute to the progress of endometriosis. These promising identified biomarkers underpin the metabolic pathway including steroid hormone biosynthesis, glycerophospholipid metabolism, sphingolipid metabolism, pyruvate metabolism, bile acid biosynthesis, and androgen and estrogen metabolisms. Considering that a much higher throughput can be obtained without a chromatographic step, the present WT-ESI-MS method could be developed as a fast prognostic or diagnostic method for endometriosis.

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endometriosis

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