Vibrational characterization of granulosa cells from patients affected by unilateral ovarian endometriosis: New insights from infrared and Raman microspectroscopy

Valentina Notarstefano, Giorgia Gioacchini, Hugh J. Byrne, Carlotta Zacà, E Sereni, Elena Sereni, Sereni E, Lisa Vaccari, Andrea Borini, Oliana Carnevali, Elisabetta Giorgini
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2019 · vol. 212 , pp. 206–214 · doi:10.1016/j.saa.2018.12.054 · PMID:30639914 · W2907323199
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Infrared and Raman microspectroscopy revealed similar protein, lipid, and carbohydrate alterations, along with oxidative stress, in granulosa cells from both ovaries of women with unilateral ovarian endometriosis.

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This study used infrared and Raman microspectroscopy to analyze luteinized granulosa cell samples collected separately from both ovaries of women with unilateral ovarian endometriosis, comparing them with granulosa cells from women with tubal, idiopathic, or male infertility. The authors reported similar biochemical alterations in granulosa cells from the two ovaries affected by unilateral endometriosis, including changes in protein patterns, indications of oxidative stress mechanisms, and deregulation of lipid and carbohydrate metabolism. A stated limitation is that the work is based on spectroscopic characterization of cells from specific patient groups rather than direct functional validation of oocyte outcomes. This paper is centrally about endometriosis — it specifically uses infrared and Raman microspectroscopy to characterize metabolic and biochemical changes in granulosa cells from unilateral ovarian endometriosis across both ovaries.

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Abstract

Endometriosis is a chronic gynaecological disease characterised by the presence of endometrial cells in extra-uterine regions. One of the main factors impacting on the fertility of women affected by endometriosis is the poor oocyte quality. Granulosa Cells (GCs) regulate oocyte development and maintain the appropriate microenvironment for the acquisition of its competence; hence, the dysregulation of these functions in GCs can lead to severe cellular damages also in oocytes. In this study, luteinized GCs samples were separately collected from both ovaries of women affected by Unilateral Ovarian Endometriosis and analysed by infrared and Raman microspectroscopy. The spectral data were compared with those of GCs from women with diagnosis of tubal, idiopathic or male infertility (taken as control group). The coupling of these two spectroscopic techniques sheds new light on the alteration induced by this pathology on GCs metabolism and biochemical composition. In fact, the study revealed similar biochemical modifications in GCs from both ovaries of women affected by unilateral ovarian endometriosis, such as the alteration of the protein pattern, the induction of oxidative stress mechanisms, and the deregulation of lipid and carbohydrate metabolisms. These evidences suggest that unilateral endometriosis impairs the overall ovarian functions, causing alterations not only in the ovary with endometriotic lesions but also in the contralateral "healthy" one.
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Document Type Article Rights Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence Disciplines 1.4 CHEMICAL SCIENCES Publication Details Spectrochimica Acta A: Molecular and Biomolecular Spectroscopy, 2019 Abstract Endometriosis is a chronic gynaecological disease characterised by the presence of endometrial cells in extra-uterine regions. One of the main factors impacting on the fertility of women affected by endometriosis is the poor oocyte quality. Granulosa Cells (GCs) regulate oocyte development and maintain the appropriate microenvironment for the acquisition of its competence; hence, the dysregulation of these functions in GCs can lead to severe cellular damages also in oocytes. In this study, luteinized GCs samples were separately collected from both ovaries of women affected by Unilateral Ovarian Endometriosis and analysed by infrared and Raman microspectroscopy. The spectral data were compared with those of GCs from women with diagnosis of tubal, idiopathic or male infertility (taken as control group). The coupling of these two spectroscopic techniques sheds new light on the alteration induced by this pathology on GCs metabolism and biochemical composition. In fact, the study revealed similar biochemical modifications in GCs from both ovaries of women affected by unilateral ovarian endometriosis, such as the alteration of the protein pattern, the induction of oxidative stress mechanisms, and the deregulation of lipid and carbohydrate metabolisms. These evidences suggest that unilateral endometriosis impairs the overall ovarian functions, causing alterations not only in the ovary with endometriotic lesions but also in the contralateral "healthy" one. DOI https://doi.org/10.1016/j.saa.2018.12.054 Recommended Citation Notarstefano, V. et al. (2019). Vibrational characterization of granulosa cells from patients affected by unilateral ovarian endometriosis: new insights from infrared and Raman microspectroscopy. Spectrochemica Acta A: Molecular and Biomolecular Spectroscopy212, pp.206-214. doi: 10.1016/j.saa.2018.12.054 Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Granulosa Cells Spectrum Analysis, Raman Vibration Endometriosis Female Granulosa Cells Humans Microspectrophotometry Multivariate Analysis Spectrum Analysis, Raman

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