Secretion of matrix metalloproteinases by human endometrial cells in vitro
Lab / animal
OA: bronze
CC0
⤵ 12 in-corpus citations
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Human endometrial stromal and epithelial cells secrete matrix metalloproteinases, whose expression depends on cell preparation purity, adhesion, and substrate.
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Abstract
At each menstrual cycle, the uterine endometrium undergoes intense remodelling. Of the many factors implicated in tissue remodelling, the matrix metalloproteinases (MMPs) play a central role owing to their capacity to degrade the extracellular matrix. The aim of this study was to examine the nature and cellular origin of endometrial proteases as evaluated in culture systems with clearly characterized cell types. Endometrial cells from hysterectomy specimens were prepared using collagenase digestion. Bone-marrow-derived cells (a known source of proteases) were removed by immunopurification. Cells were cultured on different substrates (matrigel, agarose, glass or plastic). Purity of cell preparations was examined by immunocytochemistry, and proteases were characterized by zymography on SDS-PAGE containing gelatin. The cell phenotype in culture was largely influenced by the type of substrate. Gelatin-degrading enzymes detected in culture supernatants of stromal and epithelial cells had molecular masses ranging from 42 to 248 kDa, and were identified as metalloproteinases. We conclude that human endometrial stromal and epithelial cells express several matrix metalloproteinases, the expression of which clearly depended on the purity of cell preparation, on cell adhesion and on the nature of the substrate on which the cells grew. These enzymes might be involved in endometrium remodelling, blastocyst implantation and trophoblast invasion.
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Cited by (12)
- Evaluation of ovarian cancer risk in granulosa cells treated with steroid-depleted endometriosis serum: Role of NF-κB/RelA and AKT 2018
- Deregulation of LOXL1 and HTRA1 gene expression in endometriosis 2010
- Endometrial paradigms 2008
- Expressão gênica diferencial em tecido endometrial tópico e lesões endometrióticas 2007
- Endometriosis: epidemiology and aetiological factors 2004
- Endometriosis: epidemiology and aetiological factors 2004
- Pathogenesis of endometriosis 2002
- Decreased tissue inhibitor of metalloproteinase in the endometrium of women using depot medroxyprogesterone acetate: a role for altered endometrial matrix metalloproteinase/tissue inhibitor of metalloproteinase balance in the pathogenesis of abnormal uterine bleeding? 2002
- Current concepts in the pathogenesis of endometriosis 1999
- Adhesion of human endometrium to the epithelial lining and extracellular matrix of amnion in vitro: an electron microscopic study 1998
- Suppression of matrix metalloproteinases inhibits establishment of ectopic lesions by human endometrium in nude mice 1997
- Focal cellular origin and regulation of interstitial collagenase (matrix metalloproteinase-1) are related to menstrual breakdown in the human endometrium 1996
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- openalex
- last seen: 2026-06-10T17:14:06.276822+00:00
- pubmed
- last seen: 2026-10-08T21:59:31.578486+00:00
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