MiR-664-2 impacts pubertal development in a precocious-puberty rat model through targeting the NMDA receptor-1†

Minda Ju, Yang Liu, Liu Yang, Jing Zhu, Zhejun Chen, Mizhen Zhang, Jin Yu, Zhanzhuang Tian
Biology of reproduction · 2019 · vol. 100(6) , pp. 1536–1548 · doi:10.1093/biolre/ioz044 · PMID:30916745 · W2925012153
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This study found that reduced miR-664-2 expression in rats enhances NMDAR1 signaling, leading to precocious puberty by promoting GnRH secretion.

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Abstract

Precocious puberty (PP) commonly results from premature activation of the hypothalamic-pituitary-gonadal axis (HPGA). Gonadotropin-releasing hormone (GnRH) is the initial trigger for HPGA activation and plays an important role in puberty onset. N-methyl-D-aspartate (NMDA) can promote pulsatile GnRH secretion and accelerates puberty onset. However, the mechanism of N-methyl-D-aspartate receptors (NMDARs) in PP pathogenesis remains obscure. We found that serum GnRH, luteinizing hormone (LH), follicle-stimulating hormone (FSH), estrogen (E2) levels, hypothalamic NMDAR1, and GnRH mRNA expression peaked at the vaginal opening (VO) day. Next, the hypothalamic NMDAR1 mRNA and protein levels in rats treated with danazol, a chemical commonly effecting on the reproductive system, were significantly increased at the VO day (postnatal day 24) compared to controls, accompanied by enhanced serum GnRH, LH, FSH, and E2 levels. Further, microRNA-664-2 (miR-664-2) was selected after bioinformatics analysis and approved in primary hypothalamic neurons, which binds to the 3'-untranslated regions of NMDAR1. Consistently, the miR-664-2 expression in hypothalamus of the Danazol group was decreased compared to Vehicle. Our results suggested that attenuated miR-664-2 might participate in PP pathogenesis through enhancing the NMDAR1 signaling.

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MeSH descriptors

Puberty, Precocious Receptors, N-Methyl-D-Aspartate Sexual Maturation Animals Danazol Danazol Estrogen Antagonists Estrogen Antagonists Female Gene Expression Regulation, Developmental Gene Expression Regulation, Developmental Gonadotropin-Releasing Hormone Gonadotropin-Releasing Hormone Gonadotropin-Releasing Hormone Gonadotropin-Releasing Hormone Male Puberty, Precocious Random Allocation Rats Rats, Sprague-Dawley

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