Down-regulated miR-124-3p enhanced the migration and epithelial-stromal transformation of endometrial stromal cells extracted from eutopic endometrium in subjects with adenomyosis by up-regulating Neuropilin 1

Nan Huang, Huang N, Liyan Xu, Li‐Yan Xu, Xu L, Yafen Qiu, Qiu Y, Zhan J, Jinlai Zhan, Xiangjun Chen, Chen X
Tissue & cell · 2020 · vol. 69 , pp. 101474 · doi:10.1016/j.tice.2020.101474 · PMID:33387827 · W3116203589
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This study found that reduced miR-124-3p expression in endometrial stromal cells from women with adenomyosis promoted cell migration and epithelial-stromal transition by increasing Neuropilin 1.

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Abstract

MiR-124-3p regulates the biological function of endometrial cancer cells. However, the role of miR-124-3p in adenomyosis (AM) has not been reported. Hence, we hypothesized that miR-124-3p also regulated the development of AM. The expressions of miR-124-3p and Neuropilin 1 (NRP1) in AM endometrial tissues were evaluated by Quantitative Real-time-PCR (qPCR). Endometrial stromal cells (ESCs) were isolated from the eutopic endometrial tissue of women with AM and further identified using immunofluorescence. Then the target of miR-124-3p was predicted by Starbase V2.0 and verified by dual-luciferase assay. After transfection of miR-124-3p mimic, inhibitor, or NRP1 overexpression plasmids, the viability and migration of ESCs were measured by Cell counting kit -8 (CCK-8) and wound healing assays, respectively. The expressions of NRP1 and epithelial-stromal transformation (EST)-related factors were evaluated by Quantitative Real-time-PCR (qPCR) or Western blot. MiR-124-3p was down-regulated and NRP1 was up-regulated in AM eutopic endometrial tissues. NRP1 was targeted by miR-124-3p. The extracted ESCs were Vimentin-positive and Cytokeratin-negative. MiR-124-3p mimic decreased viability, migration, and the expressions of NRP1, Vimentin, N-cadherin, and matrix metalloproteinase (MMP-9) in ESCs while increasing the expression of E-cadherin. MiR-124-3p inhibitor and NRP1 overexpression had the contrary effect of miR-124-3p on ESCs. Furthermore, NRP1 overexpression offset the effect of miR-124-3p mimic on viability, migration, and expressions of NRP1 and EMT-related factors in ESCs. MiR-124-3p regulated the migration and EMT of ESCs by down-regulating NRP1.

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Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Cell Movement Down-Regulation Endometrium Epithelial Cells MicroRNAs Neuropilin-1 Up-Regulation Adenomyosis Base Sequence Cell Movement Cell Survival Cell Survival Down-Regulation Endometrium Epithelial Cells Female Humans MicroRNAs MicroRNAs

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