The mRNA expression and DNA methylation level of fibronectin 1 (FN1) gene encoding focal adhesion molecule in endometrial endometriosis

Irwina Eka Deraya, Andon Hestiantoro, Raden Muharam, M. Luky Satria Marwali, Darmawi Darmawi, Asmarinah Asmarinah
In: IOP Conference Series: Earth and Environmental Science · 2020 · vol. 457(1) , pp. 012079 · doi:10.1088/1755-1315/457/1/012079 · W3014015407
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This study found increased fibronectin 1 (FN1) gene mRNA expression in endometriosis compared to normal endometrium, with a significant difference in promoter methylation levels, although methylation did not correlate with increased expression.

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This study investigated the role of epigenetic mechanisms in endometriosis by analyzing the mRNA expression and promoter methylation levels of the fibronectin 1 (FN1) gene. Researchers compared samples from patients with endometrial endometriosis against normal endometrial tissue using methylation-specific PCR and reverse transcription quantitative PCR. The results indicated that while FN1 mRNA expression was higher in endometriosis cases, the difference was not statistically significant, whereas the promoter methylation level showed a significant difference between the two groups. Furthermore, the analysis revealed no significant correlation between the methylation status and mRNA expression, suggesting that DNA methylation alterations did not drive the observed changes in gene expression. This paper is centrally about endometriosis — specifically examining the epigenetic regulation of focal adhesion molecules in endometrial endometriosis.

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Abstract

Abstract Endometriosis is characterized by the presence of endometrial-like tissues located outside the uterine cavity. The expression of focal adhesion genes including FN1 gene increased in endometriosis. Epigenetic factors play a role in the alteration of expression that lead to the pathological conditions. This study aimed to analyze the mRNA expression and promoter methylation level of FN1 gene as an epigenetic mechanism in endometriosis. Forty of the total samples from endometrial patients and normal were used. The DNA and RNA were isolated, DNA was converted using sodium bisulfite procedure, amplified by MSP method. Promoter methylation level was determined by intensity of the bands that arose in gel electrophoresis using ImageJ software. The RNA was reverse transcribed into cDNA and was amplified using RT-qPCR. The mRNA expression level of FN1 gene was higher in endometrial endometriosis compared to normal, but not significant statistically (p=0,63). There was a significant difference methylation level of FN1 gene in endometrial endometriosis compared to normal endometrium (p=0.022). There was positive correlation between promoter methylation level to its mRNA expression in endometrial endometriosis (r=0.080; p=0.736). DNA methylation alteration in promoter FN1 gene was not to be caused by the increasing of mRNA expression.
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Abstract Endometriosis is characterized by the presence of endometrial-like tissues located outside the uterine cavity. The expression of focal adhesion genes including FN1 gene increased in endometriosis. Epigenetic factors play a role in the alteration of expression that lead to the pathological conditions. This study aimed to analyze the mRNA expression and promoter methylation level of FN1 gene as an epigenetic mechanism in endometriosis. Forty of the total samples from endometrial patients and normal were used. The DNA and RNA were isolated, DNA was converted using sodium bisulfite procedure, amplified by MSP method. Promoter methylation level was determined by intensity of the bands that arose in gel electrophoresis using ImageJ software. The RNA was reverse transcribed into cDNA and was amplified using RT-qPCR. The mRNA expression level of FN1 gene was higher in endometrial endometriosis compared to normal, but not significant statistically (p=0,63). There was a significant difference methylation level of FN1 gene in endometrial endometriosis compared to normal endometrium (p=0.022). There was positive correlation between promoter methylation level to its mRNA expression in endometrial endometriosis (r=0.080; p=0.736). DNA methylation alteration in promoter FN1 gene was not to be caused by the increasing of mRNA expression. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

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endometriosis

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