Comparison of HSCORE assessment of endometrial beta3 integrin subunit expression with digital HSCORE using computerized image analysis (ImageJ).

Daniele G Fuhrich, Bruce A. Lessey, Ricardo Francalacci Savaris
In: Analytical and quantitative cytopathology and histopathology · 2013 · vol. 35(4) , pp. 210–6 · PMID:24341124 · PMC4090774 · W2304541820
article OA: green CC0 ⤵ 10 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed
⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

Digital HSCORE using ImageJ shows high correlation with expert traditional HSCORE for endometrial beta3 integrin expression and eliminates interobserver variation for inexperienced researchers.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

OBJECTIVE: To compare intraobserver and interobserver variation between traditional histological score (HSCORE) and digital HSCORE (D-HSCORE) performed by expert and naive researchers. STUDY DESIGN: Immunohistochemical analysis of beta3 integrin subunit of 100 endometrial biopsies obtained from the midluteal phase of the menstrual cycle were reanalyzed using ImageJ software (D-HSCORE). Mean intensity of 3,3'-diaminobenzidine on endometrial glands was read by an expert (HSCORE) versus inexperienced observer using HSCORE and D-HSCORE. RESULTS: The mean correlation [r(s)(95% CI)] between both methods was 0.86 (0.79-0.90) and highly significant (p < 0.0001) for the experienced individual. The naive researcher overestimated immunostaining, resulting (HSCORE) in negative samples. No discrepancies were seen with D-HSCORE. Interobserver variation for the inexperienced reader was 50% using HSCORE (cutoff 0.7) but 0% with D-HSCORE. Intraobserver variation using ImageJ was 0%. CONCLUSION: The D-HSCORE performed by an inexperienced researcher has high correlation to traditional HSCORE performed by an expert.

My notes (saved in your browser only)

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (30)

Cited by (10)

Source provenance

europepmc
last seen: 2026-06-11T06:19:48.454388+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK