Effects of the Novel Orally Active Antiestrogen TZE-5323 on Experimental Endometriosis

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The novel anti-estrogen TZE-5323 effectively reduced endometriosis lesion volume in rats without adverse effects on bone density or estradiol levels.

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Abstract

Danazol and gonadotropin-releasing hormone agonists which are used as therapeutic drugs for endometriosis, develop adverse reactions in association with their long-term use. The efficacy of anti-estrogens for endometriosis, an estrogen-dependent disorder, has not been demonstrated. A novel, orally active anti-estrogen, TZE-5323 ((2-cyclohexy-6-hydroxybenzo[b]thien-3-yl)[4-[2-(1- piperidinyl)ethoxy]phenyl] methanone hydrochloride, CAS 150797-71-0; free salt formula) was developed. TZE-5323 showed strong affinity for human estrogen receptor alpha (hER alpha) and beta (hER beta), and dose-dependently inhibited estradiol-stimulated transcriptional activation via hER alpha and hER beta. Furthermore, TZE-5323 dose-dependently reduced estrogen-increased uterine weight in ovariectomized rats. Tamoxifen showed agonistic activity on hER alpha, while TZE-5323 did not show such activity. In the experimental endometriosis model in rats in which endometrial tissue is autotransplanted into the renal subcapsular space, TZE-5323 dose-dependently reduced the volume of the endometrial implant as did danazol and leuprorelin acetate. Furthermore, the long-term administration of TZE-5323 neither showed a decrease in bone mineral density nor did it affect serum estradiol concentrations in intact rats. Therefore, TZE-5323 suggested its potential as a novel therapeutic drug for endometriosis which is effective also in long-term use.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Estrogen Antagonists Piperidines Animals Bone Density Bone Density Chloramphenicol O-Acetyltransferase Chloramphenicol O-Acetyltransferase Cholesterol Cholesterol Endometriosis Endometrium Endometrium Endometrium Estradiol Estradiol Estrogen Antagonists Estrogen Antagonists Estrogen Receptor alpha Estrogen Receptor beta

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europepmc
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