Aberrant Overexpression of RNA-Editing Enzyme ADAR1 Promotes the Progression of Endometriosis

Considerable efforts have been invested to elucidate the potential mechanisms involved in the physiopathology of endometriosis. However, to date, prior research has not been conclusive. This research has examined one particular mechanism, i.e., the effect of ADAR1 on endometriosis lesions. Eutopic endometrium was collected from women with (n = 25) and without endometriosis (n = 25), respectively. The expression of ADAR1 mRNA was measured based on quantitative real-time polymerase chain reactions (RT-qPCR). Both Western blot and immunohistochemistry were performed to establish ADAR1 protein expression levels. The results indicated that ADAR1 mRNA and proteins were significantly greater in the eutopic endometrium of the women with endometriosis, compared to the women without (P < 0.05). The Cell Counting Kit-8 (CCK-8) and EdU method were conducted to examine the effect of ADAR1 on cell viability and proliferation in eutopic endometriosis cells. A transwell assay was also used to detect the role of ADAR1 in the invasion of endometrial cells. The results obtained showed that ADAR1 promoted endometrial cell viability, proliferation, and invasion (P < 0.05). This informed our conclusion that the ADAR1 gene is upregulated in endometriosis, potentially paying a pivotal role in the physiopathology of endometriosis. Similar content being viewed by others

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The RNA-editing enzyme ADAR promotes lung adenocarcinoma migration and invasion by stabilizing FAK. Sci Signal. 2017:10(497). Funding The author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: This work was supported by the Henan Science and Technology Research Project of China. The work was also supported by the Department of Obstetrics and Gynecology of the Third Affiliated Hospital of Zhengzhou University. Author information Authors and Affiliations Corresponding author Ethics declarations Conflict of Interest The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. Rights and permissions About this article Cite this article Li, Z., Qi, R., Wang, Q. et al. Aberrant Overexpression of RNA-Editing Enzyme ADAR1 Promotes the Progression of Endometriosis. Reprod. Sci. 27, 575–584 (2020). https://doi.org/10.1007/s43032-019-00057-w Received: Accepted: Published: Version of record: Issue date: DOI: https://doi.org/10.1007/s43032-019-00057-w