The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of Endometriosis

Background: In endometriosis there is a decrease in endometrial receptivity. Kebar and Metformin grasses are expected to improve the condition of endometrial receptivity

To find out whether there is any effect of giving Kebar grass extract and metformin on endometrial receptivity

The study was designed by using a simple experimental randomization. It was conducted in Obstetrics Gynecology Department - Faculty of Medicine Diponegoro University and Faculty of Veterinary Medicine Airlangga University. Sample calculation was employed. The study was done within five groups including control group with normal mice, group of endometriosis mouse models given placebo, group of endometriosis mouse models given Kebar grass extract, group of endometriosis mouse models given metformin, and group of endometriosis mouse models given the combination of Kebar grass – metformin extract. The examination materials of ανβ3 integrin and Leukemia Inhibitory Factors were taken from the uteri of the mice and measured by the Rammele Scale Index (Immuno Reactive Score), while the Superoxide Dismutase examination using ELISA was derived from mice serum. These examinations were performed by two veterinarians

The expression of ανβ3 integrin was significantly higher in the group of mouse models given Kebar grass extract as well as the combination of Kebar and metformin grass extracts (p <0.05) rather than in the group of endometriosis mouse models with no treatment, whereas LIF expression was significantly higher in the group given Kebar grass extract, metformin and combination of both extracts (p <0.05). Serum SOD levels remained the same.

The expressions of ανβ3 Integrin and LIF are higher due to the provision of Kebar grass extract as well as the combination of Kebar grass and metformin extracts Note: This article has supplementary file(s). Fulltext View|Download | Research Instrument Graphics and Table Email colleagues | Subject | | | Type | Research Instrument | | Download (142KB) Indexing metadata |

Integrin ανβ3; Leukemia Inhibitory Factor; Superoxide Dismutase; Kebar grass; Metformin Article Metrics: Article Info - Greaves E, Cousins FL, Murray A, Esnal-Zufiaurre A, Fassbender A, Horne AW, et al. A Novel Mouse Model of Endometriosis Mimics Human Phenotype an Reveals Insights into the Inflammatory Contribution of Shed Endometrium. Am J Pathol. 2014;184:1930 - 9 - Titrikou S, Eklu-Gadegbeku K, Mouzou A, Aklikokou K, Gbeassor M. Calcium Antagonistic Activity of Biophytum petersianum on Vascular Smooth Muscles of Wistar Rat. IJPT. 2007;6:185 - 9 - Diana S. Potensi Tanaman Obat Banondit (Biophytum petersianum Klotzsch) Sebagai Sumber Pakan Hijauan di Lembah Kebar Papua Barat. Pastura. 2012;2:34 - 6 - Sembiring B, Darwati I. Identification fo chemical component of Kebar grass accession (Biophytum petersianum) from Papua and Java. Bul Littro. 2014;25:37 - 44 - Sadsoetoeboen PD. Manfaat Estrak Rumput Kebar (Biophytum petersianum Klotzch) Terhadap Penampilan Reproduksi Mencit Putih Betina. Bogor: Institut Pertanian Bogor; 2005 - Sukarsono S, Istianah L, Lestari EU, Aminin ALN. Effect of grass Kebar (biophytum petersianum klotszch) to increase uterus fertility and increasing number of children on female white rat (rattus norvegicus). Int J Fertil Steril. 2012;6:137 - Foda AA, Aal IAA. Metformin as new therapy for endometriosis, its effects on both clinical picture and cytokines profile. Middle East Fertil Soc J. 2012:262 - 7 - Xu JN, Zeng C, Zhou Y, Peng C, Zhou YF, Xue Q. Metformin Inhibits StAR Expression in Human Endometriotic Stromal Cells via AMPK-Mediated Disruption of CREB-CRTC2 Complex Formation. J Clin Endocrinol Metab. 2014;99(8):2795 - 803 - Oner G, Ozcelik B, Ozgun MT, Serin IS, Ozturk F, Basbug M. The effects of metformin and letrozole on endometriosis and comparison of the two treatment agents in a rat model. Hum Reprod. 2010;25(4):932 - 7 - Sag D, Carling D, Stout RD, Suttles J. AMP-activated protein kinase promotes macrophage polarization to an anti-inflammatory functional phenotype. J Immunol. 2008;181(12):8633 - 41 - Sahra IB, Regazzetti C, Robert G, Laurent K, Marchand-Brustel YL, Auberger P, et al. Metformin, Independent of AMPK, Induces mTOR Inhibiton and Cell-Cycle Arrest through REDD1. Cancer Res. 2011;71(13):4386 -72 - Aisemberg J, Vercelli CA, Bariani MV, Billi SC, Wolfson ML, Franchi AM. Progesterone is Essential for Protecting against LPS-Induced Pregnancy Loss. LIF as a Potential Mediator of the Anti-inflammatory Effect of Progesteron. PLoS One. 2013;8(2):e56161 - Park JH, Lee SK, Kim MK, Lee JH, Yun BH, Park JH, et al. Saponin Extracts Induced Apoptosis of Endometrial Cells From Women With Endometriosis Through Modulation of miR-21-5p. Reprod Sci. 2017;24:53 - 60 - Bulun SE, Cheng YH, Pavone ME, Yin P, Imir G, Utsunomiya H, et al. 17β-Hydroxysteroid Dehydrogenase-2 Deficiency and Progesterone Resistance in Endometriosis. Semin Reprod Med. 2010;28(1):44 - 50 - Yu WJ, Lee BJ, Nam SY, Yang DC, Yun YW. Modulating Effects of Korean Ginseng Saponins on Ovarian Function in Immature Rats. Bio Pharm Bull. 2003;26(11):1574 - 80 - Revel A. Defective endometrial receptivity. Fertil Steril. 2012;97(5):1028 - 32 - Bulun SE, Monsavais D, Pavone ME, Dyson M, Xue Q, Attar E, et al. Role of Estrogen Receptor-β in Endometriosis. Semin Reprod Med. 2012;30(1):39 - 45 - Trukhacheva E, Lin Z, Reierstad S, Cheng YH, Milad M, Bulun SE. Estrogen Receptor (ER)β Regulates ERα Expression in Stromal Cells Derived from Ovarian Endometriosis. J Clin Endocrinol Metab. 2009;94(2):615 - 22 - Unitly AJA, Inara C, editors. Potensi Rumput Kebar (Biophytum petersianum Klotzsch) Dalam Meningkatkan Kinerja Reproduksi. Seminar Nasional Pengembangan Pulau-Pulau Kecil 2011; 2011; Bogor - Oepomo TD, editor. Endometriosis: Patogenesis, Dampak pada Kualitas Hidup, Penanggulangan. 1 ed. Surakarta: Sebelas Maret University Press; 2015 - Sakhthivel K.M GC. Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis. Pharm Biol. 2014;52(12):1570 - 80 - Abhirama B, Rajagopal SS, Nanjan M. Nephroprotective effect of ethanol extract of Biophytum sensitivum (Linn.) DC in cisplatin-induced experimental renal damage in rats. Research J Pharm and Tech. 2017;10(6):1772 - 9 - Guney M, Oral B, Karahan N, Mungan T. Regression of endometrial explants in a rat model of endometriosis treated with the melatonin. Fertil Steril. 2008;89(4):934 - 42 - Pekel A, Gonenc A, Turhan NO, Kafah H. Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF. J Assist Reprod Genet. 2015;32:233 - 41 Last update: No citation recorded. Last update: No citation recorded. Copyright (c) 2018 Journal of Biomedicine and Translational Research The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Journal of Biomedicine and Translational Research Diponegoro University as publisher of the journal. Copyright encompasses non-exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. Journal of Biomedicine and Translational Research Diponegoro University, the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Journal of Biomedicine and Translational Research Diponegoro University (JBTR) are sole and exclusive responsibility of their respective authors and advertisers. The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form JBTR] The copyright form should be signed originally and send to the Editorial Office in the form of original mail, scanned document or fax : Journal of Biomedicine and Translational Research Faculty of Medicine, Diponegoro University Jl. Prof. Soedarto, Kampus UNDIP Tembalang, Semarang, Central Java, Indonesia 50275, Telp.: +62-24-8454714, Fax.: +62-24-8454714 Email: [email protected]