Metabolic changes in myometrium associated with adenomyosis revealed by liquid chromatography-tandem mass spectrometry-based high-throughput targeted lipid quantitative profiling

Qian Liang, Liang Qian, Gensheng Wang, G. Wang, Xuejiao Chen, Hao Chen, Qing Li, Feng Chen, Wenjun Liu, Wenhua Liu, Dan Wang, Honglin Xu, Hongling Xu, Ziqiang Qian, Dingheng Li
Reproductive biomedicine online · 2025 · vol. 51(2) , pp. 104861 · doi:10.1016/j.rbmo.2025.104861 · PMID:40472664 · W4407382387
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⚙ AI-generated summary by claude@2026-06, 2026-06-06 ⓘ

This study used liquid chromatography–tandem mass spectrometry to identify metabolic alterations in the myometrium of women with adenomyosis.

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Abstract

RESEARCH QUESTION: What are the lipidomic alterations in the myometrial tissue from adenomyotic lesions? DESIGN: This was a two-centre, prospective, observational study conducted between July 2023 and June 2024. Forty-four premenopausal patients were enrolled: 22 patients with adenomyosis confirmed by post-operative pathology and 22 control participants undergoing hysterectomy for benign conditions without adenomyosis. Myometrial tissue samples were collected during surgery, and lipid extraction performed by liquid-liquid extraction and lipidomic profiling carried out using a liquid chromatography-tandem mass spectrometry platform. Data analysis included multivariate statistical methods, such as principal component analysis and orthogonal partial least squares discriminant analysis, to identify lipid metabolites differentially expressed between the adenomyosis and control groups. RESULTS: A total of 1100 lipid species were identified, with 84 showing significant differences between adenomyosis patients and control participants. Of these, 76 lipids were upregulated and eight were down-regulated. The highest differentially expressed lipid content was mainly concentrated in glyceropholipids, with notable alterations in acylcarnitines and lysophosphatidylcholines. CONCLUSION: This study reveals significant lipidomic changes in adenomyosis, with upregulated acylcarnitines and elevated lysophosphatidylcholines.

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Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Lipid Metabolism Lipid Metabolism Lipid Metabolism Lipid Metabolism Lipid Metabolism Lipid Metabolism Lipid Metabolism Lipid Metabolism

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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