Immunohistochemical Parameters of Musashi-1 in Nodular and Diffuse Adenomyosis

I. F. Kozachenko, K. M. Dzhamalutdinova, Dzhamalutdinova KM, Н. М. Файзуллина, Faizullina NM, N M Faizullina, Shchegolev AI, А. И. Щеголев, A I Shchegolev
Bulletin of experimental biology and medicine · 2017 · vol. 163(4) , pp. 506–509 · doi:10.1007/s10517-017-3839-2 · PMID:28853072 · W2752903730
article OA: closed CC0 ⤵ 3 in-corpus citations
Full text JSON View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite+body, 2026-06-13 ⓘ

Musashi-1 expression, indicating proliferative potential, was enhanced in adenomyosis foci compared to eutopic endometrium, with the highest staining observed in nodular adenomyosis epithelial cells during the secretory phase.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-06, 2026-06-13 · read from full text ⓘ

This study used immunohistochemistry to compare the expression of the somatic stem-cell marker Musashi-1 in eutopic endometrium across menstrual cycle phases and in patients with nodular versus diffuse adenomyosis, quantifying nuclear and cytoplasmic optical density in epithelial and stromal components. Musashi-1 staining in eutopic endometrium was more intensive during the proliferative phase than the secretory phase, while adenomyosis foci showed enhanced Musashi-1 expression compared with endometrial cells, with the strongest staining in nodular adenomyosis, particularly in epithelial cells during the secretory phase. The paper’s main limitation is that it is descriptive and comparative at the protein-staining level without assessing functional mechanisms underlying the observed differences. This paper is centrally about adenomyosis—specifically, Musashi-1 immunohistochemical differences between nodular and diffuse adenomyosis and their relation to menstrual-phase changes in eutopic endometrium.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Comparative immunohistochemical analysis of the expression of somatic stem cells marker Musashi-1 in the endometrium was performed during various phases of menstrual cycle and in patients with nodular and diffuse adenomyosis. The expression of Musashi-1 reflecting proliferative potential of epithelial and stromal cells was quantitatively analyzed by the optical density in the nuclei and cytoplasm of epithelial and stromal cells of the eutopic endometrium and adenomyosis foci. In the eutopic endometrium, the immunohistochemical reaction was more intensive during proliferation phase in comparison with secretion phase. Enhanced expression of Musashi-1 was observed in adenomyosis foci in comparison with endometrial cells. The most intensive staining was found in nodular adenomyosis, especially in epithelial cells during the secretion phase. These data attest to the role of somatic stem cells in the development and progression of various forms of adenomyosis.
Full text 4,332 characters · extracted from oa-doi-fallback · click to expand
Comparative immunohistochemical analysis of the expression of somatic stem cells marker Musashi-1 in the endometrium was performed during various phases of menstrual cycle and in patients with nodular and diffuse adenomyosis. The expression of Musashi-1 reflecting proliferative potential of epithelial and stromal cells was quantitatively analyzed by the optical density in the nuclei and cytoplasm of epithelial and stromal cells of the eutopic endometrium and adenomyosis foci. In the eutopic endometrium, the immunohistochemical reaction was more intensive during proliferation phase in comparison with secretion phase. Enhanced expression of Musashi-1 was observed in adenomyosis foci in comparison with endometrial cells. The most intensive staining was found in nodular adenomyosis, especially in epithelial cells during the secretion phase. These data attest to the role of somatic stem cells in the development and progression of various forms of adenomyosis. Similar content being viewed by others References Adamyan LV, Kulakov VI, Andreeva EN. Endometriosis. Moscow, 2006. Russian. Shklyar AA, Adamyan LV, Kogan EA, Paramonova NB, Kozachenko IF, Gavrilova TYu, Kononov SN. Difficulties in diagnosing nodular and diffuse adenomyosis. Akush. Ginekol. 2015(3):67-72. Russian. Shchegolev AI, Bykov AG, Tumanova UN, Pavlovich SV. Endometriosis and the development of tumors. Akush. Ginekol. 2016(11):49-56. Russian. Brenner RM, Slayden OD, Rodgers WH, Critchley HO, Carroll R, Nie XJ, Mah K. Immunocytochemical assessment of mitotic activity with an antibody to phosphorylated histone H3 in the macaque and human endometrium. Hum. Reprod. 2003;18(6):1185-1193. Chen YZ, Wang JH, Yan J, Liang Y, Zhang XF, Zhou F. Increased expression of the adult stem cell marker Musashi-1 in the ectopic endometrium of adenomyosis does not correlate with serum estradiol and progesterone levels. Eur. J. Obstet. Gynecol. Reprod. Biol. 2014;173:88-93. Fox RG, Park FD, Koechlein CS, Kritzik M, Reya T. Musashi signaling in stem cells and cancer. Annu. Rev. Cell Dev. Biol. 2015;31:249-267. Götte M, Wolf M, Staebler A, Buchweitz O, Kelsch R, Schüring AN, Kiesel L. Increased expression of the adult stem cell marker Musashi-1 in endometriosis and endometrial carcinoma. J. Pathol. 2008;215(3):317-329. Harper JA, Yuan JS, Tan JB, Visan I, Guidos CJ. Notch signaling in development and disease. Clin. Genet. 2003;64(6):461-472. Kim CM, Oh YJ, Cho SH, Chung DJ, Hwang JY, Park KH, Cho DJ, Choi YM, Lee BS. Increased telomerase activity and human telomerase reverse transcriptase mRNA expression in the endometrium of patients with endometriosis. Hum. Reprod. 2007;22(3):843-849. Ma L, Xu YL, Ding WJ, Shao HF, Teng YC. Prognostic value of Musashi-1 in endometrioid adenocarcinoma. Int. J. Clin. Exp. Pathol. 2015;8(5):4564-4572. Nguyen MS, Voci S. Adenomyomatosis. Ultrasound Q. 2013;29(3):215-217. Nnoaham KE, Hummelshoj L, Webster P, d’Hooghe T, de Cicco Nardone F, de Cicco Nardone C, Jenkinson C, Kennedy SH, Zondervan KT; World Endometriosis Research Foundation Global Study of Women’s Health consortium. Impact of endometriosis on quality of life and work productivity: a multicenter study across ten countries. Fertil. Steril. 2011;96(2):366-373. Okano H, Kawahara H, Toriya M, Nakao K, Shibata S, Imai T. Function of RNA binding protein Musashi-1 in stem cells. Exp. Cell. Res. 2005;306(2):349-356. Schwab KE, Chan RW, Gargett CE. Putative stem cell activity of human endometrial epithelial and stromal cells during the menstrual cycle. Fertil. Steril. 2005;84(Suppl. 2):1124-1130. Siddall NA, McLaughlin EA, Marriner NL, Hime GR. The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity. Proc. Natl. Acad. Sci. USA. 2006;103(22):8402-8407. Author information Authors and Affiliations Corresponding author Additional information Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 4, pp. 509-513, April, 2017 Rights and permissions About this article Cite this article Kozachenko, I.F., Dzhamalutdinova, K.M., Faizullina, N.M. et al. Immunohistochemical Parameters of Musashi-1 in Nodular and Diffuse Adenomyosis. Bull Exp Biol Med 163, 506–509 (2017). https://doi.org/10.1007/s10517-017-3839-2 Received: Published: Issue date: DOI: https://doi.org/10.1007/s10517-017-3839-2

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

⚙ Ask this paper AI returns verbatim quotes from the full text · source: oa-doi-fallback ⓘ

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Immunohistochemistry Nerve Tissue Proteins RNA-Binding Proteins Adenomyosis Adult Cell Nucleus Cell Nucleus Cytoplasm Cytoplasm Endometrium Endometrium Female Humans Immunohistochemistry Menstrual Cycle Menstrual Cycle Middle Aged Nerve Tissue Proteins RNA-Binding Proteins

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (13)

Cited by (3)

Source provenance

europepmc
last seen: 2026-08-24T06:08:07.662257+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:20:19.560968+00:00
unpaywall
last seen: 2026-08-24T06:27:14.670421+00:00
License: CC0 · commercial use OK