{"paper_id":"142dd8b4-3e42-4cf1-9d09-99266ba9a6b6","body_text":"Comparative immunohistochemical analysis of the expression of somatic stem cells marker Musashi-1 in the endometrium was performed during various phases of menstrual cycle and in patients with nodular and diffuse adenomyosis. The expression of Musashi-1 reflecting proliferative potential of epithelial and stromal cells was quantitatively analyzed by the optical density in the nuclei and cytoplasm of epithelial and stromal cells of the eutopic endometrium and adenomyosis foci. In the eutopic endometrium, the immunohistochemical reaction was more intensive during proliferation phase in comparison with secretion phase. Enhanced expression of Musashi-1 was observed in adenomyosis foci in comparison with endometrial cells. The most intensive staining was found in nodular adenomyosis, especially in epithelial cells during the secretion phase. These data attest to the role of somatic stem cells in the development and progression of various forms of adenomyosis.\nSimilar content being viewed by others\nReferences\nAdamyan LV, Kulakov VI, Andreeva EN. Endometriosis. Moscow, 2006. Russian.\nShklyar AA, Adamyan LV, Kogan EA, Paramonova NB, Kozachenko IF, Gavrilova TYu, Kononov SN. Difficulties in diagnosing nodular and diffuse adenomyosis. Akush. Ginekol. 2015(3):67-72. Russian.\nShchegolev AI, Bykov AG, Tumanova UN, Pavlovich SV. Endometriosis and the development of tumors. Akush. Ginekol. 2016(11):49-56. Russian.\nBrenner RM, Slayden OD, Rodgers WH, Critchley HO, Carroll R, Nie XJ, Mah K. Immunocytochemical assessment of mitotic activity with an antibody to phosphorylated histone H3 in the macaque and human endometrium. Hum. Reprod. 2003;18(6):1185-1193.\nChen YZ, Wang JH, Yan J, Liang Y, Zhang XF, Zhou F. Increased expression of the adult stem cell marker Musashi-1 in the ectopic endometrium of adenomyosis does not correlate with serum estradiol and progesterone levels. Eur. J. Obstet. Gynecol. Reprod. Biol. 2014;173:88-93.\nFox RG, Park FD, Koechlein CS, Kritzik M, Reya T. Musashi signaling in stem cells and cancer. Annu. Rev. Cell Dev. Biol. 2015;31:249-267.\nGötte M, Wolf M, Staebler A, Buchweitz O, Kelsch R, Schüring AN, Kiesel L. Increased expression of the adult stem cell marker Musashi-1 in endometriosis and endometrial carcinoma. J. Pathol. 2008;215(3):317-329.\nHarper JA, Yuan JS, Tan JB, Visan I, Guidos CJ. Notch signaling in development and disease. Clin. Genet. 2003;64(6):461-472.\nKim CM, Oh YJ, Cho SH, Chung DJ, Hwang JY, Park KH, Cho DJ, Choi YM, Lee BS. Increased telomerase activity and human telomerase reverse transcriptase mRNA expression in the endometrium of patients with endometriosis. Hum. Reprod. 2007;22(3):843-849.\nMa L, Xu YL, Ding WJ, Shao HF, Teng YC. Prognostic value of Musashi-1 in endometrioid adenocarcinoma. Int. J. Clin. Exp. Pathol. 2015;8(5):4564-4572.\nNguyen MS, Voci S. Adenomyomatosis. Ultrasound Q. 2013;29(3):215-217.\nNnoaham KE, Hummelshoj L, Webster P, d’Hooghe T, de Cicco Nardone F, de Cicco Nardone C, Jenkinson C, Kennedy SH, Zondervan KT; World Endometriosis Research Foundation Global Study of Women’s Health consortium. Impact of endometriosis on quality of life and work productivity: a multicenter study across ten countries. Fertil. Steril. 2011;96(2):366-373.\nOkano H, Kawahara H, Toriya M, Nakao K, Shibata S, Imai T. Function of RNA binding protein Musashi-1 in stem cells. Exp. Cell. Res. 2005;306(2):349-356.\nSchwab KE, Chan RW, Gargett CE. Putative stem cell activity of human endometrial epithelial and stromal cells during the menstrual cycle. Fertil. Steril. 2005;84(Suppl. 2):1124-1130.\nSiddall NA, McLaughlin EA, Marriner NL, Hime GR. The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity. Proc. Natl. Acad. Sci. USA. 2006;103(22):8402-8407.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nAdditional information\nTranslated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 4, pp. 509-513, April, 2017\nRights and permissions\nAbout this article\nCite this article\nKozachenko, I.F., Dzhamalutdinova, K.M., Faizullina, N.M. et al. Immunohistochemical Parameters of Musashi-1 in Nodular and Diffuse Adenomyosis. Bull Exp Biol Med 163, 506–509 (2017). https://doi.org/10.1007/s10517-017-3839-2\nReceived:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s10517-017-3839-2","source_license":"CC0","license_restricted":false}