Analisis Efektivitas Nanocurcumin Terhadap Ekspresi TNF-α Dan Diameter Lesi Pada Mencit (Mus musculus) Yang Diinduksi Endometriosis

Komang Ayu Vanessa Dharma, Florence Pribadi, Irwin Priyatna Kusumah
In: Journal of Pharmaceutical and Sciences · 2026 · pp. 18–27 · doi:10.36490/journal-jps.com.v9i1.1249 · W7118658415
article OA: diamond CC0
🔓 Open OA copy Full text JSON View on OpenAlex View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-07-16 ⓘ

This study found that nanocurcumin significantly reduced endometriosis lesion size in mice, but did not significantly alter TNF-α levels.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-07, 2026-07-16 · read from full text ⓘ

This study evaluated nanocurcumin (10 mg/kg body weight) in endometriosis-induced mice to assess effects on serum TNF-α expression and on endometriosis lesion diameter, using a post-test only control group design with three groups: nanocurcumin administered after induction, nanocurcumin administered before induction, and an untreated control (n=10 per group). TNF-α was quantified by ELISA and lesion diameter was measured macroscopically, with statistical testing including Shapiro–Wilk, Levene’s test, ANOVA, and Tukey’s extended test. Nanocurcumin produced a significant reduction in lesion diameter (p < 0.001) with the greatest lesion shrinkage in the nanocurcumin group, but TNF-α levels did not differ significantly between groups (p = 0.933). The paper relates to endometriosis because it directly tests nanocurcumin’s impact on inflammatory cytokine expression and lesion size in an endometriosis mouse model. This paper is centrally about endometriosis — it analyzes nanocurcumin’s effects on TNF-α expression and endometriosis lesion diameter in mice.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Endometriosis merupakan penyakit ginekologi kronis yang ditandai oleh pertumbuhan jaringan mirip endometrium di luar uterus, yang memicu inflamasi lokal dan progresi lesi. Proses patologisnya melibatkan peningkatan sitokin proinflamasi, termasuk TNF-α, serta aktivasi jalur proliferatif dan angiogenik. Nanocurcumin, dengan bioavailabilitas yang lebih tinggi dibandingkan kurkumin konvensional, diketahui memiliki potensi modulasi inflamasi dan penghambatan pertumbuhan jaringan ektopik. Penelitian ini bertujuan untuk menganalisis pengaruh nanocurcumin dosis 10 mg/kgBB terhadap ekspresi TNF-α dan diameter lesi endometriosis pada mencit yang diinduksi endometriosis. Penelitian eksperimental laboratorium menggunakan desain post-test only control group dengan tiga kelompok perlakuan (kontrol, nanocurcumin setelah induksi, dan nanocurcumin sebelum induksi), masing-masing terdiri dari 10 mencit. Ekspresi TNF-α diukur menggunakan metode ELISA, sedangkan diameter lesi dinilai secara makroskopis. Analisis data dilakukan menggunakan uji normalitas Shapiro–Wilk, homogenitas Levene, ANOVA, dan uji lanjut Tukey. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan bermakna kadar TNF-α antar kelompok (p = 0,933). Namun, terdapat perbedaan signifikan pada diameter lesi endometriosis (p < 0,001), di mana kelompok nanocurcumin menunjukkan penyusutan lesi paling besar dibandingkan kelompok kontrol dan kelompok perlakuan lainnya. Nanocurcumin secara signifikan mengurangi diameter lesi endometriosis pada mencit, meskipun tidak menurunkan kadar TNF-α serum secara signifikan. Temuan ini menunjukkan bahwa efek terapeutik nanocurcumin mungkin dimediasi melalui mekanisme lokal yang tidak sepenuhnya tercermin dalam biomarker serum,
Full text 5,095 characters · extracted from oa-doi-fallback · 2 sections · click to expand

Abstract

Endometriosis is a chronic gynecological disease characterized by the growth of endometrial-like tissue outside the uterus, which triggers local inflammation and lesion progression. The pathological process involves increased proinflammatory cytokines, including TNF-α, and activation of proliferative and angiogenic pathways. Nanocurcumin, with higher bioavailability compared to conventional curcumin, is known to have the potential to modulate inflammation and inhibit ectopic tissue growth. This study aimed to analyze the effect of nanocurcumin at a dose of 10 mg/kgBW on TNF-α expression and endometriosis lesion diameter in endometriosis-induced mice. The laboratory experimental study used a post-test only control group design with three treatment groups (control, nanocurcumin after induction, and nanocurcumin before induction), each consisting of 10 mice. TNF-α expression was measured using the ELISA method, while lesion diameter was assessed macroscopically. Data analysis was performed using the Shapiro–Wilk normality test, Levene's homogeneity test, ANOVA, and Tukey's extended test. The results showed no significant difference in TNF-α levels between groups (p = 0.933). However, there was a significant difference in endometriosis lesion diameter (p < 0.001), with the nanocurcumin group showing the greatest lesion shrinkage compared to the control and other treatment groups. Nanocurcumin significantly reduced the diameter of endometriosis lesions in mice, although it did not significantly reduce serum TNF-α levels. These findings suggest that the therapeutic effects of nanocurcumin may be mediated through local mechanisms that are not fully reflected in serum biomarkers. Downloads Article Details This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

References

C. M. Becker et al., ‘ESHRE guideline: endometriosis’, Human Reproduction, vol. 1, no. 1, 1–26, pp. 1–6. B. Smolarz, K. Szyłło, and H. Romanowicz, ‘ Endometriosis: Epidemiology, classification, pathogenesis, treatment and genetics (review of literature)’, Int J Mol Sci 22., 2021. DOI: https://doi.org/10.3390/ijms221910554 F. G. Martire et al., ‘Endometriosis and Adenomyosis : From Pathogenesis to Follow-Up’, Curr Issues Mol Biol, vol. 47, pp. 1–8, 2025. DOI: https://doi.org/10.3390/cimb47050298 WHO, ‘Endometriosis [WWW Document]’, 2023. D. Khairani, S. Ilyas, and Yurnadi, Prinsip Dan Praktik Hewan Percobaan Mencit (Mus musculus). Medan: USU Press, 2024. R. Kapoor, C. A. Stratopoulou, and M. M. Dolmans, ‘Pathogenesis of endometriosis: New insights into prospective therapies ’, Int J Mol Sci, vol. 22, pp. 1–14, 2021. DOI: https://doi.org/10.3390/ijms222111700 P. K. Wardani, D. R. Gunarti, and Y. Wulandari, ‘Peran Flavonoid Terhadap Tnf Alpha Pada Endometriosis’, Jurnal Darma Agung, vol. 31, p. 241, 2023. DOI: https://doi.org/10.46930/ojsuda.v31i3.3454 A. Karthikeyan, N. Senthil, and T. Min, ‘Nanocurcumin: A Promising Candidate for Therapeutic Applications’, Front Pharmacol, vol. 11, 2020. DOI: https://doi.org/10.3389/fphar.2020.00487 E. P. Hestianah, W. Widjiati, J. M. Ntoruru, and M. Y. A. Widyanugraha, ‘Administration of Nanocurcumin in Mice Models of Endometriosis as an Effort to Improve Folliculogenesis’, Biomedical and Pharmacology Journal, vol. 17, pp. 939–947, 2024. DOI: https://doi.org/10.13005/bpj/2914 Z. Liu, J. D. Smart, and A. S. Pannala, ‘Recent developments in formulation design for improving oral bioavailability of curcumin: A review’, J Drug Deliv Sci Technol, 2020. DOI: https://doi.org/10.1016/j.jddst.2020.102082 F. Sharifipour, S. F. Siahkal, K. Qaderi, Z. Mohaghegh, M. Zahedian, and F. Azizi, ‘Effect of Curcumin on Dysmenorrhea and Symptoms of Premenstrual Syndrome: A Systematic Review and Meta-Analysis’, Korean J Fam Med, vol. 96, pp. 96–104, 2024. DOI: https://doi.org/10.4082/kjfm.23.0184 D. Firmansyah and Dede, ‘Teknik Pengambilan Sampel Umum dalam Metodologi’, Jurnal Ilmiah Pendidikan Holistik (JIPH), vol. 1, pp. 85–114, 2022. DOI: https://doi.org/10.55927/jiph.v1i2.937 M. D. T. Lala and D. R. Sari, ‘Perbandingan Gambaran Histologis Lapisan Piramidalis Area Ca1 Hipokampus Mus Musculus yang Dipapar Radiasi Gelombang Elektromagnetik Telepon Seluler 3G dan 4G’, Jurnal Penelitian Perawat Profesional, vol. 5, pp. 1279–1290, 2023. DOI: https://doi.org/10.37287/jppp.v5i3.1772 A. Vallée and Y. Lecarpentier, ‘Curcumin and endometriosis’, Int J Mol Sci, vol. 21, pp. 1–18, 2020. DOI: https://doi.org/10.3390/ijms21072440 J. Ding, S. Mei, W. Cheng, Z. Ni, and C. Yu, ‘Curcumin treats endometriosis in mice by the HIF signaling pathway’, Am J Transl Res, vol. 14, pp. 2184–2198, 2022. S. Y. Kim, Y. Y. Kyaw, M. S. Seong, K. H. Kim, and J. Cheong, ‘Curcumin suppresses an endometrial cell inflammation through inhibition of SREBP-1’, Integr Mol Med, vol. 6, pp. 1–6, 2019. DOI: https://doi.org/10.15761/IMM.1000384

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

⚙ Ask this paper AI returns verbatim quotes from the full text · source: oa-doi-fallback ⓘ

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Condition tags

endometriosis

Citation neighborhood (sparse)

Too few in-corpus citations on either side for a chart; here are the lists.

Cites (4)

References (12)

Source provenance

openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK