Human Endometrium Derived Mesenchymal Stem Cells with Aberrant NOD1 Expression Are Associated with Ectopic Endometrial Lesion Formation

Chunmei Li, Suiyu Luo, Ai Guo, Ying Su, Yuhui Zhang, Yan Song, Mei Liu, L Y Wang, Yuanyuan Zhang
International journal of stem cells · 2024 · vol. 17(3) , pp. 309–318 · doi:10.15283/ijsc22200 · PMID:38531608 · PMC11361846 · W4394623743
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Endometriosis-derived mesenchymal stem cells exhibit higher NOD1 expression, and NOD1 activation enhances proliferation and invasion, suggesting its role in ectopic lesion formation.

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Abstract

Nucleotide-binding oligomerization domain 1 (NOD1), a cytosolic pattern recognition receptor protein, plays a crucial role in innate immune responses. However, the functional expression of NOD1 in mesenchymal stem cells (MSCs) derived from endometriosis remains unclear. The aim of this study was to explore the functions of NOD1 in ectopic endometrial lesions. Tissues and MSCs were isolated from both normal endometrium and endometriosis. Immunohistochemistry and real time quantitative polymerase chain reaction (RT-qPCR) were used to determine the expression of NOD1 in the tissues/MSCs. Quantification of various cytokines was performed using RT-qPCR and enzyme-linked immunosorbent assay. To confirm the proliferation, invasion/migration, and apoptotic viabilities of the samples, Cell Counting Kit-8, clonogenic formation, transwell assays, and apoptotic experiments were conducted. Higher levels of NOD1 expression were detected in the ectopic-MSCs obtained from endometriosis compared to those from the endometrium. The expression of interleukin-8 was higher in the ectopic-MSCs than in the eutopic-MSCs. Pretreatment with NOD1 agonist significantly enhanced the proliferation and invasion/migration of eutopic-MSCs. Additionally, the NOD1 inhibitor ML-130 significantly reduced the proliferation, clone formation, invasion, and migration abilities of the ectopic-MSCs, having no effect on their apoptosis capacity. Our findings suggest that the expression of NOD1 in ectopic-MSCs may contribute to the progression of ectopic endometrial lesions.

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Condition tags

endometriosis

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References (25)

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organisms 12
human mus sp. mammals human naine d'afrique de l'ouest rabbits human rabbits transgenic mice human transgenic mice human
chemicals 27
formaldehyde formaldehyde xylene ethanol hydrogen peroxide n-[(2r,3r,4r,5s,6r)-2-[[(2r,3r,4r,5r,6s)-5-acetamido-6-[(2r,3r,4s,5s,6r)-2-[(2r,3r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,3s,4r,5r,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3-hydroxy-4-[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]methoxy]-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide haematoxylin penicillin streptomycin cocaethylene oil red o ruthenium red brilliant blue pyrimidinedicarboxylic acid water iodonitrotetrazolium salt triton phenylindole polystyrene polymer propidium iodide alexa fluor 488 peptide glycan gelatin progesterone

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