Preventive effects of clomiphene citrate on estrogen-deficiency osteopenia elicited by LHRH agonist administration in the rat

A Goulding, Ailsa Goulding, Goulding A, Fisher L, L Fisher, Lorryn Fisher
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 1991 · vol. 6(11) , pp. 1177–1182 · doi:10.1002/jbmr.5650061106 · PMID:1805541 · W2086978918
article OA: closed CC0 ⤵ 3 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-11 ⓘ

Clomiphene citrate administration prevented bone loss induced by the LHRH agonist buserelin in rats by slowing bone resorption, without altering buserelin's hypoestrogenic effects.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

The estrogen agonist and antagonist clomiphene citrate has been shown to prevent bone loss induced by ovariectomy in the rat. In young women estrogen-deficiency bone loss is a clinical problem associated with the use of luteinizing hormone releasing hormone (LHRH) agonists, such as buserelin, to treat endometriosis. The aim of this study was to determine whether clomiphene citrate (10 mg/kg body weight per week orally) would prevent the osteopenic effect of buserelin (25 micrograms/kg body weight per day SC) in the rat. Four groups of animals with 45Ca-labeled skeletons were studied for 4 weeks: group A, placebo controls; group B, buserelin; group C, clomiphene; and group D, buserelin + clomiphene. Bone resorption was monitored by measuring the urinary excretion of 45Ca and hydroxyproline. Clomiphene slowed bone breakdown and inhibited the osteopenic effect of buserelin. Total-body calcium values (mean +/- SD) were (mg) 2635 +/- 181, 2267 +/- 85, 2566 +/- 126, and 2624 +/- 77 in groups A to D, respectively. Osteopenia was present only in group B (P less than 0.001). Interestingly buserelin lowered blood 17 beta-estradiol and uterine weights to a similar extent in the presence and absence of clomiphene. Because clomiphene inhibited estrogen-deficiency bone loss in buserelin-treated rats without depressing the hypoestrogenic action of this LHRH agonist, it is suggested that the use of clomiphene to protect the skeleton during LHRH agonist therapy of endometriosis warrants further study.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Bone Diseases, Metabolic Bone Resorption Buserelin Clomiphene Estrogens Animals Bone Diseases, Metabolic Bone Diseases, Metabolic Bone Diseases, Metabolic Bone Resorption Bone Resorption Buserelin Calcium Calcium Clomiphene Endometriosis Endometriosis Estrogens Estrogens Female

Citation neighborhood (sparse)

Too few in-corpus citations on either side for a chart; here are the lists.

Cites (4)

Cited by (3)

References (25)

Cited by (3)

Source provenance

europepmc
last seen: 2026-08-13T06:15:24.848197+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:11:54.876058+00:00
unpaywall
last seen: 2026-08-13T06:47:16.638238+00:00
License: CC0 · commercial use OK